Nie Weidan, Liu Lili, Chen Yinxia, Luo Mingyin, Feng Chenghao, Wang Chaonan, Yang Zhongmin, Du Chong
College of Horticulture, Xinjiang Agricultural University, Urumqi 830052, China.
Plants (Basel). 2023 Jun 22;12(13):2416. doi: 10.3390/plants12132416.
Root-knot nematode (RKN) infections are among the most serious soil-borne diseases in the world, and tomato is a common host of RKNs. WRKY transcription factors are involved in complex, diverse biological processes in plants. In a previous study, a resistant variety, LA3858 (/), was treated at different soil temperatures before RNA-seq, and six differentially expressed genes (DEGs) encoding WRKY proteins were screened. In this study, cloning and sequencing were used to identify six target DEGs encoding SlWRKY1, SlWRKY13, SlWRKY30, SlWRKY41, SlWRKY46, and SlWRKY80. Conserved domain identification and phylogenetic tree analysis showed that SlWRKY1, SlWRKY13, and SlWRKY46 have similar functions and are mainly involved in plant growth and development and abiotic stress responses. SlWRKY30 and SlWRKY41 share high homology, while AtWRKY46 and AtWRKY70, which are highly homologous to SlWRKY80, play an important role in the disease resistance of . Considering these findings combined with the high level of expression observed in the roots and leaves of the resistant variety Motelle (/) and the continuous upregulation of expression in the roots after inoculation of Motelle with , it is speculated that plays an important role in the -mediated disease resistance pathway. Further study revealed that SlWRKY80 is a typical nuclear-localized protein, and a virus-induced gene silencing (VIGS) assay verified that is involved in tomato resistance to RKNs as a positive regulator. SA and JA signals play an important role in -mediated resistance to RKNs. was able to respond rapidly to treatment with both plant hormones, which indicated that might be involved in disease resistance regulation through various immune pathways.
根结线虫（RKN）感染是世界上最严重的土传病害之一，番茄是根结线虫的常见寄主。WRKY转录因子参与植物复杂多样的生物学过程。在先前的一项研究中，一个抗性品种LA3858（/）在进行RNA测序之前在不同土壤温度下处理，筛选出了6个编码WRKY蛋白的差异表达基因（DEGs）。在本研究中，利用克隆和测序鉴定了6个编码SlWRKY1、SlWRKY13、SlWRKY30、SlWRKY41、SlWRKY46和SlWRKY80的目标DEGs。保守结构域鉴定和系统发育树分析表明，SlWRKY1、SlWRKY13和SlWRKY46具有相似功能，主要参与植物生长发育和非生物胁迫响应。SlWRKY30和SlWRKY41具有高度同源性，而与SlWRKY80高度同源的AtWRKY46和AtWRKY70在（此处原文缺失信息）的抗病性中起重要作用。结合这些发现以及在抗性品种Motelle（/）的根和叶中观察到的高水平（此处原文缺失信息）表达以及Motelle接种（此处原文缺失信息）后根中（此处原文缺失信息）表达的持续上调，推测（此处原文缺失信息）在（此处原文缺失信息）介导的抗病途径中起重要作用。进一步研究表明，SlWRKY80是一种典型的核定位蛋白，病毒诱导基因沉默（VIGS）试验证实（此处原文缺失信息）作为正向调节因子参与番茄对根结线虫的抗性。水杨酸（SA）和茉莉酸（JA）信号在（此处原文缺失信息）介导的对根结线虫的抗性中起重要作用。（此处原文缺失信息）能够对两种植物激素的处理迅速做出反应，这表明（此处原文缺失信息）可能通过各种免疫途径参与抗病调节。
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