College of Horticulture, Xinjiang Agricultural University, Urumqi 830052, China.
Postdoctoral Station of Horticulture, Xinjiang Agricultural University, Urumqi 830052, China.
Int J Mol Sci. 2024 Aug 15;25(16):8892. doi: 10.3390/ijms25168892.
WRKY transcription factors (TFs) can participate in plant biological stress responses and play important roles. was found to be differentially expressed in the - and -resistant tomato lines by RNA-seq and may serve as a key node for disease resistance regulation. This study used RNAi to determine whether silencing could influence the sensitivity of 'M82' (/)-susceptible lines to . Further overexpression of this gene revealed a significant increase in tomato disease resistance, ranging from highly susceptible to susceptible, combined with the identification of growth (plant height, stem diameter, and leaf area) and physiological (soluble sugars and proteins; root activity) indicators, clarifying the role of as a positive regulatory factor in tomato defense against . Based on this phenomenon, a preliminary exploration of its metabolic signals revealed that stimulates different degrees of signaling, such as salicylic acid (SA), jasmonic acid (JA), and ethylene (ETH), and may synergistically regulate reactive oxygen species (ROS) accumulation and scavenging enzyme activity, hindering the formation of feeding sites and ultimately leading to the reduction of root gall growth. To our knowledge, has an extremely high utilization value for improving tomato resistance to root-knot nematodes and breeding.
WRKY 转录因子（TFs）可以参与植物生物胁迫反应，并发挥重要作用。通过 RNA-seq 发现， 在 抗虫和抗虫番茄品系中差异表达，可能作为抗病调控的关键节点。本研究利用 RNAi 技术确定沉默 是否会影响 'M82'（-/）敏感品系对 的敏感性。进一步过表达该基因，发现番茄抗病性显著增强，从高度敏感到敏感，同时鉴定出生长（株高、茎直径和叶面积）和生理（可溶性糖和蛋白质；根活力）指标，阐明 作为番茄防御根结线虫的正调控因子的作用。基于这一现象，对其代谢信号进行了初步探索，发现 刺激不同程度的信号，如水杨酸（SA）、茉莉酸（JA）和乙烯（ETH），并可能协同调节活性氧（ROS）积累和清除酶活性，阻碍取食位点的形成，最终导致根结生长减少。据我们所知， 对于提高番茄抗根结线虫能力和育种具有极高的利用价值。
