College of Plant Protection, Northwest A&F University, Yangling 712100, China.
College of Plant Science, Tibet Agricultural and Animal Husbandry University, Nyingchi 860000, China.
Int J Mol Sci. 2023 Jan 30;24(3):2583. doi: 10.3390/ijms24032583.
Tomato powdery mildew, caused by , is a destructive fungal disease that damages almost all of the aerial parts of tomato, causing devastating losses in tomato production worldwide. WRKY transcription factors are key regulators of plant immunity, but the roles of in wild tomato LA1777 against still remain to be uncovered. Here, we show that is an important WRKY transcription factor from wild tomato LA1777, contributing to plant resistance against . was isolated and identified to positively modulate tomato resistance against -Lz. The transient overexpression of the ShWRKY81-GFP (green fluorescent protein) fusion protein in cells revealed that ShWRKY81 was localized in the nucleus. responded differentially to abiotic and biotic stimuli, with mRNA accumulation in LA1777 seedlings upon -Lz infection. The virus-induced gene silencing of led to host susceptibility to -Lz in LA1777, and a loss of HO formation and hypersensitive response (HR) induction. Furthermore, the transcripts of were induced by salicylic acid (SA), and silenced LA1777 seedlings displayed decreased levels of the defense hormone SA and SA-dependent gene expression upon -Lz infection. Together, these results demonstrate that acts as a positive player in tomato powdery mildew resistance.
番茄白粉病由 引起，是一种破坏性真菌病害，几乎损害番茄的所有地上部分，导致番茄在世界范围内减产。WRKY 转录因子是植物免疫的关键调节剂，但 在野生番茄 LA1777 中对 的作用仍有待揭示。在这里，我们表明 是来自野生番茄 LA1777 的一个重要 WRKY 转录因子，有助于植物对 的抗性。 被分离并鉴定为正向调控番茄对 -Lz 的抗性。ShWRKY81-GFP（绿色荧光蛋白）融合蛋白在 细胞中的瞬时过表达表明 ShWRKY81 定位于细胞核中。 对非生物和生物胁迫有不同的反应，在 LA1777 幼苗中，-Lz 感染后 mRNA 的积累。在 LA1777 中，通过病毒诱导的基因沉默使 沉默导致对 -Lz 的易感性，并导致 HO 形成和过敏反应（HR）诱导的丧失。此外， 的转录物被水杨酸（SA）诱导，沉默的 LA1777 幼苗在 -Lz 感染后表现出防御激素 SA 和 SA 依赖性 基因表达水平降低。总之，这些结果表明 作为番茄白粉病抗性的正向参与者发挥作用。
