Yang Lang, Liu Jinhong, Wong Ching-Kiu, Lim Boon Leong
School of Biological Sciences, University of Hong Kong, Pokfulam, Hong Kong, China.
State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, China.
Plants (Basel). 2023 Jun 29;12(13):2489. doi: 10.3390/plants12132489.
The growth of pollen tubes, which depends on actin filaments, is pivotal for plant reproduction. Pharmacological experiments showed that while oryzalin and brefeldin A treatments had no significant effect on the lipid droplets (LDs) trafficking, while 2,3-butanedione monoxime (BDM), latrunculin B, SMIFH2, and cytochalasin D treatments slowed down LDs trafficking, in such a manner that only residual wobbling was observed, suggesting that trafficking of LDs in pollen tube is related to F-actin. While the trafficking of LDs in the wild-type pollen tubes and in , , , and single mutants and / double mutant were normal, their trafficking slowed down in a myosin-XI double knockout (/) mutant. These observations suggest that Myo11C1 and Myo11C2 motors are involved in LDs movement in pollen tubes, and they share functional redundancy. Hence, LDs movement in Arabidopsis pollen tubes relies on the actomyosin system.
依赖于肌动蛋白丝的花粉管生长对植物繁殖至关重要。药理学实验表明，虽然oryzalin和布雷菲德菌素A处理对脂滴（LDs）运输没有显著影响，但2,3-丁二酮单肟（BDM）、拉特罗毒素B、SMIFH2和细胞松弛素D处理减缓了LDs运输，以至于仅观察到残余的摆动，这表明花粉管中LDs的运输与F-肌动蛋白有关。虽然野生型花粉管以及 、 、 和 单突变体和 / 双突变体中LDs的运输正常，但在肌球蛋白-XI双敲除（ / ）突变体中其运输减缓。这些观察结果表明，Myo11C1和Myo11C2马达参与花粉管中LDs的运动，并且它们具有功能冗余。因此，拟南芥花粉管中LDs的运动依赖于肌动球蛋白系统。
