不同光谱组成的光对欧洲赤松的抗氧化状态、某些色素和次生代谢产物含量以及相关基因表达的影响
Effect of Light of Different Spectral Compositions on Pro/Antioxidant Status, Content of Some Pigments and Secondary Metabolites and Expression of Related Genes in Scots Pine.
作者信息
Pashkovskiy Pavel, Ivanov Yury, Ivanova Alexandra, Kartashov Alexander, Zlobin Ilya, Lyubimov Valery, Ashikhmin Aleksandr, Bolshakov Maksim, Kreslavski Vladimir, Kuznetsov Vladimir, Allakhverdiev Suleyman I
机构信息
K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia.
Institute of Basic Biological Problems, Russian Academy of Sciences, Institutskaya Street 2, 142290 Pushchino, Russia.
出版信息
Plants (Basel). 2023 Jul 5;12(13):2552. doi: 10.3390/plants12132552.
The aim of this study was to investigate the effect of light quality (white fluorescent light, WFL, containing UV components), red light (RL, 660 nm), blue light (BL, 450 nm), and white LED light (WL, 450 + 580 nm) on the components of the cellular antioxidant system in L. in needles, roots, and hypocotyls, focusing on the accumulation of key secondary metabolites and the expression of related genes. The qualitative and quantitative composition of carotenoids; the content of the main photosynthetic pigments, phenolic compounds, flavonoids (catechins, proanthocyanidins, anthocyanins), ascorbate, and glutathione; the activity of the main antioxidant enzymes; the content of hydrogen peroxide; and the intensity of lipid peroxidation (MDA and 4-HNE contents) were determined. RL resulted in an increase in the content of hydrogen peroxide and 4-HNE, as well as the total fraction of flavonoids in the needles. It also enhanced the expression of several (pathogen-related) genes compared to BL and WL. WFL increased the content of phenols, including flavonoids, and enhanced the overall activity of low-molecular antioxidants in needles and hypocotyls. BL increased the content of ascorbate and glutathione, including reduced glutathione, in the needles and simultaneously decreased the activity of peroxidases. Thus, by modifying the light quality, it is possible to regulate the accumulation of secondary metabolites in pine roots and needles, thereby influencing their resistance to various biotic and abiotic stressors.
本研究旨在探讨光质（含紫外线成分的白色荧光灯，WFL）、红光（RL，660 nm）、蓝光（BL，450 nm）和白色发光二极管光（WL，450 + 580 nm）对樟子松针叶、根和下胚轴中细胞抗氧化系统成分的影响，重点关注关键次生代谢产物的积累及相关基因的表达。测定了类胡萝卜素的定性和定量组成；主要光合色素、酚类化合物、黄酮类化合物（儿茶素、原花青素、花青素）、抗坏血酸和谷胱甘肽的含量；主要抗氧化酶的活性；过氧化氢的含量；以及脂质过氧化强度（丙二醛和4 - 羟基壬烯醛含量）。红光导致针叶中过氧化氢和4 - 羟基壬烯醛含量增加，以及黄酮类化合物的总含量增加。与蓝光和白色发光二极管光相比，红光还增强了几种（病程相关）基因的表达。白色荧光灯增加了包括黄酮类化合物在内的酚类含量，并增强了针叶和下胚轴中低分子抗氧化剂的整体活性。蓝光增加了针叶中抗坏血酸和谷胱甘肽（包括还原型谷胱甘肽）的含量，同时降低了过氧化物酶的活性。因此，通过改变光质，可以调节松树根和针叶中次生代谢产物的积累，从而影响它们对各种生物和非生物胁迫的抗性。
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