Pashkovskiy Pavel, Khalilova Lyudmila, Vereshchagin Mikhail, Voronkov Alexander, Ivanova Tatiana, Kosobryukhov Anatoliy A, Allakhverdiev Suleyman I, Kreslavski Vladimir D, Kuznetsov Vladimir V
K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow, 127276, Russia.
Institute of Basic Biological Problems, Russian Academy of Sciences, Institutskaya Street 2, Pushchino, Moscow Region, 142290, Russia.
Plant Physiol Biochem. 2023 Oct;203:108044. doi: 10.1016/j.plaphy.2023.108044. Epub 2023 Sep 25.
Marchantia polymorpha is a convenient model for studying light of different spectral compositions on various physiological and biochemical processes because its photoreceptor system is vastly simplified. The influence of red light (RL, 660 nm), far-red light (FRL, 730 nm), blue light (BL, 450 nm), and green light (GL, 525 nm) compared to white light (high-pressure sodium light (HPSL), white LEDs (WL 450 + 580 nm) and white fluorescent light (WFL) on photosynthetic and transpiration rates, photosystem II (PSII) activity, photomorphogenesis, and the expression of light and hormonal signaling genes was studied. The ultrastructure of the chloroplasts in different tissues of the gametophyte M. polymorpha was examined. FRL led to the formation of agranal chloroplasts (in the epidermis and the chlorenchyma) with a high starch content (in the parenchyma), which led to a reduced intensity of photosynthesis. BL increased the transcription of genes for the biosynthesis of secondary metabolites - chalcone synthase (CHS), cellulose synthase (CELL), and L-ascorbate peroxidase (APOX3), which is consistent with the increased activity of low-molecular weight antioxidants. FRL increased the expression of phytochrome apoprotein (PHY) and cytokinin oxidase (CYTox) genes, but the expression of the phytochrome interacting factor (PIF) gene decreased, which was accompanied by a significant change in gametophyte morphology. Analysis of crosstalk gene expression, and changes in morphology and photosynthetic activity was carried out.
多歧藻苔是研究不同光谱组成的光对各种生理和生化过程影响的便捷模型，因为其光受体系统大大简化。研究了红光（RL，660nm）、远红光（FRL，730nm）、蓝光（BL，450nm）和绿光（GL，525nm）与白光（高压钠灯（HPSL）、白色发光二极管（WL 450 + 580nm）和白色荧光灯（WFL））相比，对光合速率、蒸腾速率、光系统II（PSII）活性、光形态建成以及光和激素信号基因表达的影响。检查了多歧藻苔配子体不同组织中叶绿体的超微结构。远红光导致无基粒叶绿体（在表皮和叶肉中）的形成，且淀粉含量高（在薄壁组织中），这导致光合作用强度降低。蓝光增加了次生代谢物生物合成相关基因——查尔酮合酶（CHS）、纤维素合酶（CELL）和L -抗坏血酸过氧化物酶（APOX3）的转录，这与低分子量抗氧化剂活性增加一致。远红光增加了光敏色素脱辅基蛋白（PHY）和细胞分裂素氧化酶（CYTox）基因的表达，但光敏色素相互作用因子（PIF）基因的表达降低，这伴随着配子体形态的显著变化。对基因表达的串扰、形态和光合活性的变化进行了分析。
