Department of Physical Education and Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria, Campus Universitario de Tafira s/n, 35017 Las Palmas de Gran Canaria, Spain.
Clinical Genetics Unit, Complejo Hospitalario Universitario Insular-Materno Infantil de Las Palmas de Gran Canaria, 35016 Las Palmas de Gran Canaria, Spain.
Nutrients. 2023 Jun 23;15(13):2848. doi: 10.3390/nu15132848.
Zynamite PX, a mango leaf extract combined with quercetin, enhances exercise performance by unknown molecular mechanisms. Twenty-five volunteers were assigned to a control (17 males) or supplementation group (8 males, receiving 140 mg of Zynamite + 140 mg quercetin/8 h for 2 days). Then, they performed incremental exercise to exhaustion (IE) followed by occlusion of the circulation in one leg for 60 s. Afterwards, the cuff was released, and a 30 s sprint was performed, followed by 90 s circulatory occlusion (same leg). muscle biopsies were obtained at baseline, 20 s after IE (occluded leg) and 10 s after Wingate (occluded leg), and bilaterally at 90 s and 30 min post exercise. Compared to the controls, the Zynamite PX group showed increased basal protein expression of Thr-CaMKIIδ (2-fold, = 0.007) and Ser-GSK3β (1.3-fold, = 0.005) and a non-significant increase of total NRF2 (1.7-fold, = 0.099) and Ser-NRF2 (1.2-fold, = 0.061). In the controls, there was upregulation with exercise and recovery of total NRF2, catalase, glutathione reductase, and Thr-CaMKIIδ (1.2-2.9-fold, all < 0.05), which was not observed in the Zynamite PX group. In conclusion, Zynamite PX elicits muscle signaling changes in resting skeletal muscle resembling those described for exercise training and partly abrogates the stress kinases responses to exercise as observed in trained muscles.
Zynamite PX，一种芒果叶提取物与槲皮素的组合，通过未知的分子机制增强运动表现。25 名志愿者被分配到对照组（17 名男性）或补充组（8 名男性，每天接受 140 毫克 Zynamite + 140 毫克槲皮素/8 小时，持续 2 天）。然后，他们进行递增运动至力竭（IE），然后用止血带阻断一条腿的循环 60 秒。之后，释放止血带，进行 30 秒冲刺，然后再进行 90 秒循环阻断（同一条腿）。在基线、IE 后 20 秒（阻断腿）和 Wingate 后 10 秒（阻断腿）以及运动后 90 秒和 30 分钟时，从双侧肌肉活检。与对照组相比，Zynamite PX 组在静息状态下 Thr-CaMKIIδ 的蛋白表达增加（2 倍， = 0.007）和 Ser-GSK3β（1.3 倍， = 0.005），并且总 NRF2（1.7 倍， = 0.099）和 Ser-NRF2（1.2 倍， = 0.061）略有增加。在对照组中，总 NRF2、过氧化氢酶、谷胱甘肽还原酶和 Thr-CaMKIIδ 的表达在运动和恢复时上调（1.2-2.9 倍，均 < 0.05），而 Zynamite PX 组则没有观察到这种情况。总之，Zynamite PX 在静息骨骼肌中引起类似于运动训练的肌肉信号变化，并部分阻断了在训练有素的肌肉中观察到的运动应激激酶反应。
