Department of Public Health Medicine, Medical School, University of Pécs, H-7624 Pécs, Hungary.
Doctoral School of Health Sciences, Faculty of Health Science, University of Pécs, H-7621 Pécs, Hungary.
Nutrients. 2023 Jun 28;15(13):2946. doi: 10.3390/nu15132946.
In recent years, artificial additives, especially synthetic food colorants, were found to demonstrate wider properties compared to their natural equivalents; however, their health impact is still not totally mapped. Our study aimed to determine the long-term (30 and 90 days) exposure effect of one of the commonly used artificial food colorants, tartrazine, on NMRI mice. The applied dose of tartrazine referred to the human equivalent dose for acceptable daily intake (ADI). Further, we evaluated its impact on the transcription of a range of epigenetic effectors, members of the DNA methyltransferase () as well as histone deacetylase ( families. Following the exposure, organ biopsies were collected from the lungs, kidneys, liver, and spleen, and the gene expression levels were determined by real-time quantitative reverse transcription PCR (RT-qPCR). Our results demonstrated significant upregulation of genes in the tested organs in various patterns followed by the intake of tartrazine on ADI. Since and genes are involved in different steps of carcinogenesis, have roles in the development of neurological disorders and the effect of dose of everyday exposure is rarely studied, further investigation is warranted to study these possible associations.
近年来，人工添加剂，特别是合成食用色素，被发现具有比天然等同物更广泛的特性；然而，它们对健康的影响仍未完全确定。我们的研究旨在确定常用人工食用色素之一，柠檬黄，对 NMRI 小鼠的长期（30 天和 90 天）暴露效应。柠檬黄的应用剂量是指可接受的每日摄入量（ADI）的人体等效剂量。此外，我们评估了它对一系列表观遗传效应物的转录的影响，包括 DNA 甲基转移酶（）和组蛋白去乙酰化酶（）家族的成员。暴露后，从肺、肾、肝和脾中采集器官活检，并通过实时定量逆转录聚合酶链反应（RT-qPCR）测定基因表达水平。我们的结果表明，在摄入 ADI 剂量的柠檬黄后，测试器官中的基因以不同的模式显著上调。由于 和 基因参与了癌症发生的不同阶段，在神经发育障碍的发展中具有作用，而且日常暴露剂量的影响很少被研究，因此有必要进一步研究这些可能的关联。
