Kuru Cansu İlke, Ulucan-Karnak Fulden, Akgol Sinan
Department of Biochemistry, Faculty of Science, Ege University, 35100 Izmir, Turkey.
Biotechnology Department, Graduate School of Natural and Applied Sciences, Ege University, 35100 Izmir, Turkey.
Polymers (Basel). 2023 Jun 27;15(13):2832. doi: 10.3390/polym15132832.
We developed selective and relatively low-cost metal-chelated nanoparticle systems for the removal of the penicillin G (Pen G) antibiotic, presented for the first time in the literature. In the nanosystem, poly(glycidyl methacrylate) nanoparticles were synthesized by a surfactant-free emulsion polymerization method and covalently bound with a tridentate-chelating ligand, iminodiacetic acid, based on the immobilized metal chelate affinity technique. It was modified with Cu, a chelating metal, to make Pen G specific. Metal-chelated nanoparticles were characterized by Fourier-transform infrared spectroscopy, energy dispersive spectrometry, zeta dimensional analysis, and scanning electron microscopy technology. Optimization studies of the Pen G removal were conducted. As a result of this study, Pen G removal with the p(GMA)-IDA-Cu nanoparticle reached its maximum adsorption capacity of 633.92 mg/g in the short time of 15 min. The Pen G adsorption of p(GMA)-IDA-Cu was three times more than that of the p(GMA) nanoparticles and two times more than that of the ampicillin adsorption. In addition, there was no significant decrease in the adsorption capacity of Pen G resulting from the repeated adsorption-desorption process of metal-chelated nanoparticles over five cycles. The metal-chelated nanoparticle had an 84.5% ability to regain its ability to regenerate the product with its regeneration capability, making the widespread use of the system very convenient in terms of reducing cost, an important factor in removal processes.
我们开发了用于去除青霉素G（Pen G）抗生素的选择性且成本相对较低的金属螯合纳米颗粒系统，这在文献中是首次提出。在该纳米系统中，通过无表面活性剂乳液聚合法合成了聚甲基丙烯酸缩水甘油酯纳米颗粒，并基于固定化金属螯合亲和技术与三齿螯合配体亚氨基二乙酸共价结合。用螯合金属铜对其进行修饰，使其对Pen G具有特异性。通过傅里叶变换红外光谱、能量色散光谱、zeta尺寸分析和扫描电子显微镜技术对金属螯合纳米颗粒进行了表征。对Pen G的去除进行了优化研究。这项研究的结果是，p（GMA）-IDA-Cu纳米颗粒在15分钟的短时间内达到了其最大吸附容量633.92 mg/g。p（GMA）-IDA-Cu对Pen G的吸附量是p（GMA）纳米颗粒的三倍，是氨苄青霉素吸附量的两倍。此外，金属螯合纳米颗粒在五个循环的重复吸附-解吸过程中，Pen G的吸附容量没有显著下降。金属螯合纳米颗粒具有84.5%的再生能力，能够恢复其再生产物的能力，这使得该系统在降低成本方面的广泛应用非常便利，而成本是去除过程中的一个重要因素。
