基于适配体的磁性纳米探针，用于通过便携式读数器对牛奶中的氯霉素进行定量检测。
Aptamer-Based Magnetic Nanoprobe for Quantitative Measurement of Chloramphenicol in Milk through Portable Reader.
作者信息
Marofi Hossein, Rafati Adele, Gill Pooria
机构信息
Department of Medical Nanotechnology, Faculty of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
The Health of Plant and Livestock Products Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
出版信息
J Med Signals Sens. 2023 May 29;13(2):136-143. doi: 10.4103/jmss.jmss_177_21. eCollection 2023 Apr-Jun.
BACKGROUND
We described here an aptamer-based magnetic nanoprobe for measuring the amount of chloramphenicol (CAP) in milk.
METHODS
The nanoprobe presented in this method consists of a magnetic nanoparticle conjugated to a specific CAP aptamer. If the target is detected in the sample, the nanoprobe binds to it, and the aptamer forms a G-quadruplex structure. This structure mimics the peroxidase activity in the presence of the hemin cofactor. If tetramethylbenzidine is added to the sample containing the nanoprobe, a blue color light is observed. After adding a stop reagent solution, the color produced is measured by a microplate reader and a portable meter.
RESULTS
This study proves a 99% positive linear relationship between the microplate reader's results and the portable meter results.
CONCLUSION
Conjugation of the aptamer to magnetic nanoparticles and applying magnetic separation operations change the nanoprobe performance by 11% for both mentioned devices.
背景
我们在此描述了一种基于适配体的磁性纳米探针，用于测量牛奶中氯霉素（CAP）的含量。
方法
该方法中呈现的纳米探针由与特定CAP适配体共轭的磁性纳米颗粒组成。如果在样品中检测到目标物，纳米探针就会与之结合，适配体形成G-四链体结构。在血红素辅因子存在的情况下，这种结构模拟过氧化物酶活性。如果将四甲基联苯胺添加到含有纳米探针的样品中，会观察到蓝色光。加入终止试剂溶液后，用酶标仪和便携式仪表测量产生的颜色。
结果
本研究证明酶标仪结果与便携式仪表结果之间存在99%的正线性关系。
结论
适配体与磁性纳米颗粒的共轭以及应用磁分离操作使上述两种设备的纳米探针性能均改变了11%。
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