Roushani Mahmoud, Rahmati Zeinab, Hoseini S Jafar, Hashemi Fath Roghayeh
Department of Chemistry, Ilam University, Ilam, Iran.
Department of Chemistry, Ilam University, Ilam, Iran.
Colloids Surf B Biointerfaces. 2019 Nov 1;183:110451. doi: 10.1016/j.colsurfb.2019.110451. Epub 2019 Aug 21.
In this research work, a biosensor with a dual recognition system was fabricated and founded on a combination of aptasensing and the molecular imprinting union of the chloramphenicol (CAP) selective detection. CAP, is an antibiotic, was applied in veterinary and human in order to treat gram-positive and gram-negative infections. It is worth mentioning that CAP residue brings about earnest side effects on human health. According to this, in this sensing system, 3-aminomethyl pyridine functionalized graphene oxide (GO) (3-ampy-RGO) has been coated on the surface of GCE. Afterwards, the silver nanoparticle (AgNPs) was coated on the 3-ampy-RGO/GCE and, then, the CAP complex-amino-aptamer (NH-Apt[CAP]) was attached to the AgNP/3-ampy-RGO/GCE using a kind of bonding formation of Ag-N. In this sense, it is worth noting that the resorcinol electropolymerization around the complex of aptamer/CAP would confine the complex and, then, retain the aptamer. Following the CAP removal, the MIP cavity, as it was supposed, synergistically acted with that of the embedded aptamer in order to construct a nanohybrid receptor. Interestingly, the double exact property of the molecular imprinting polymers and aptamers led to the superb sensing properties. In the mentioned system it was illustrated that the linear range was from 1.0 pM to 1.0 nM with the detection limit of 0.3 pM; consequently, as observed, it was better than or as good as other similar assays. Moreover, the mentioned system whose activity was observed in the various interferences presence showed great selectivity in detected the CAP. Finally, the designed sensor exhibited outstanding results when applied to detect CAP in milk samples.
在本研究工作中，基于适体传感与氯霉素（CAP）选择性检测的分子印迹结合，制备并构建了一种具有双重识别系统的生物传感器。CAP是一种抗生素，用于兽医和人类治疗革兰氏阳性和阴性感染。值得一提的是，CAP残留会对人类健康产生严重的副作用。据此，在该传感系统中，3-氨甲基吡啶功能化氧化石墨烯（GO）（3-ampy-RGO）被包覆在玻碳电极（GCE）表面。之后，银纳米颗粒（AgNPs）被包覆在3-ampy-RGO/GCE上，然后，CAP复合氨基适体（NH-Apt[CAP]）通过一种Ag-N键合形成附着在AgNP/3-ampy-RGO/GCE上。从这个意义上说，值得注意的是，围绕适体/CAP复合物的间苯二酚电聚合会限制该复合物，进而保留适体。去除CAP后，正如所设想的，分子印迹空穴与嵌入的适体协同作用，以构建一种纳米杂交受体。有趣的是，分子印迹聚合物和适体的双重精确特性导致了卓越的传感性能。在上述系统中表明，线性范围为1.0 pM至1.0 nM，检测限为0.3 pM；因此，如所观察到的，它优于或等同于其他类似检测方法。此外，上述在各种干扰存在下仍能观察到活性的系统在检测CAP时表现出很高的选择性。最后，所设计的传感器在应用于检测牛奶样品中的CAP时显示出优异的结果。
