Kato Shinya
Radioisotope Experimental Facility, Advanced Science Research Promotion Center, Mie University, Tsu, Mie 514-8507, Japan.
Med Int (Lond). 2023 Jun 26;3(4):33. doi: 10.3892/mi.2023.93. eCollection 2023 Jul-Aug.
Neuroblastoma is a typical solid tumor common in childhood. The present study investigated the inhibitory effects of lactoferrin on the proliferation of IMR-32 neuroblastoma cells, including under X-ray irradiation. In controlled assays, it was found that lactoferrin inhibited cell proliferation, accompanied by cell membrane disruption. Furthermore, intracellular reactive oxygen species generation increased in IMR-32 cells treated with lactoferrin, causing membrane lipid peroxidation and the leakage of lactate dehydrogenase. The IC values for cell proliferation were ~2.0 nM for doxorubicin, 2.7 mM for dibutyryl-cAMP and 45.9 µM for lactoferrin. X-ray irradiation at 1 Gy decreased cell proliferation to ~30%, which was not restored by lactoferrin. In the Fenton reaction system with iron chloride, lactoferrin increased hydroxyl radical (OH·) formation via HO, as confirmed by electron spin resonance spectra. On the whole, the findings of the present study indicate that lactoferrin, found abundantly in milk, may help prevent or treat neuroblastoma in infants with modest efficacy, and does not exert a protective effect against X-rays.
神经母细胞瘤是一种常见于儿童期的典型实体瘤。本研究调查了乳铁蛋白对IMR-32神经母细胞瘤细胞增殖的抑制作用，包括在X射线照射下的作用。在对照试验中，发现乳铁蛋白抑制细胞增殖，并伴有细胞膜破坏。此外，用乳铁蛋白处理的IMR-32细胞内活性氧生成增加，导致膜脂质过氧化和乳酸脱氢酶泄漏。阿霉素的细胞增殖IC值约为2.0 nM，二丁酰环磷腺苷为2.7 mM，乳铁蛋白为45.9 µM。1 Gy的X射线照射使细胞增殖降至约30%，乳铁蛋白无法使其恢复。在含有氯化铁的芬顿反应体系中，电子自旋共振光谱证实乳铁蛋白通过HO增加了羟基自由基（OH·）的形成。总体而言，本研究结果表明，在牛奶中大量存在的乳铁蛋白可能有助于以适度的疗效预防或治疗婴儿神经母细胞瘤，但对X射线没有保护作用。
