Kato Shinya
Radioisotope Experimental Facility, Advanced Science Research Promotion Center, Mie University, Tsu, Mie 514-8507, Japan.
Med Int (Lond). 2022 May 31;2(3):19. doi: 10.3892/mi.2022.44. eCollection 2022 May-Jun.
Nicotine is an alkaloid found in tobacco leaves. Smoking prevention has been a neglected issue in psychiatry; nicotine intake in conjunction with the administration of the mood stabilizer, lithium carbonate (LiCO), may negatively affect brain cells. The present study investigated the combined effects of nicotine and its metabolite, cotinine, and LiCO compared to acetylcholine and dopamine in U-251MG human glioblastoma cells. Cell proliferation was found to be decreased by nicotine and to be further suppressed following treatment with LiCO, accompanied by mitotic catastrophe and increased levels of superoxide anion radicals. By contrast, cotinine did not exert such detrimental effects. It was also found that acetylcholine did not suppress cell proliferation, whereas dopamine in conjunction with LiCO decreased cell proliferation in a concentration-dependent manner. The nicotine-induced cell growth inhibition was restored by mecamylamine, a non-competitive antagonist of nicotinic acetylcholine receptors. On the whole, the findings of the present study suggest that nicotine combined with LiCO leads to the suppression of the proliferation of human glioblastoma cells accompanied by mitotic catastrophe and superoxide anion radical generation. These findings may provide further cellular biological insight into the risks associated with smoking under LiCO administration.
尼古丁是一种存在于烟草叶中的生物碱。预防吸烟在精神病学领域一直是一个被忽视的问题；摄入尼古丁并同时服用情绪稳定剂碳酸锂（LiCO）可能会对脑细胞产生负面影响。本研究调查了尼古丁及其代谢物可替宁与LiCO相比，在U - 251MG人胶质母细胞瘤细胞中与乙酰胆碱和多巴胺的联合作用。发现尼古丁会降低细胞增殖，在用LiCO处理后会进一步受到抑制，同时伴有有丝分裂灾难和超氧阴离子自由基水平升高。相比之下，可替宁并未产生此类有害影响。还发现乙酰胆碱不会抑制细胞增殖，而多巴胺与LiCO联合使用会以浓度依赖的方式降低细胞增殖。尼古丁诱导的细胞生长抑制可被美加明恢复，美加明是一种烟碱型乙酰胆碱受体的非竞争性拮抗剂。总体而言，本研究结果表明，尼古丁与LiCO联合使用会导致人胶质母细胞瘤细胞增殖受到抑制，同时伴有有丝分裂灾难和超氧阴离子自由基生成。这些发现可能为LiCO给药情况下吸烟相关风险提供进一步的细胞生物学见解。
