Nor Islan, Wirasutisna Komar Ruslan, Hartati Rika, Insanu Muhamad
Departement of Pharmaceutical Biology, School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia.
Faculty of Pharmacy, University of Muhammadiyah Banjarmasin, Banjarmasin, Indonesia.
Saudi Pharm J. 2023 Aug;31(8):101677. doi: 10.1016/j.jsps.2023.06.010. Epub 2023 Jun 19.
Diabetes Mellitus is the main cause of death on a global scale. In 2019, there were 463 million people with diabetes, and WHO predicts that by 2030, there will be 578 million. As an antidiabetic agent, α-glucosidase inhibitors are one of the methods employed to reduce the prevalence of diabetes. Diabetes is traditionally treated with as a primary material, medicine, fruit, ornamental plant, and source of carpentry. This investigation aimed to examine the inhibitory effect of seven species of against α-glucosidase enzyme using an assay and isolate active substances and ascertain their concentrations in each sample. As a solvent, ethanol was used in maceration to extract the substance. Afterward, the extract underwent a series of fractionation techniques, including liquid-liquid extraction, vacuum liquid chromatography, column chromatography, and preparative Thin Layer Chromatography (TLC) for purification and isolation. The compound's structures were elucidated using TLC, UV-Visible spectrophotometry, and nuclear magnetic resonance (NMR) spectroscopy. Based on concentrations of 100 and 200 µg/mL, exhibited the most significant inhibitory effect, followed by other species of . The proportion of ethyl acetate had the strongest activity (IC 0.40 ± 0.02 µg/mL) contrasted to positive control acarbose (IC 55.39 ± 0.67 g/mL) and quercitrin (IC 6.47 ± 0.40 µg/mL). Avicularin and 4-O-methyl gallic acid were discovered in the ethyl acetate fraction of with IC values of 17.05 ± 0.75 µg/mL and 25.19 ± 0.21 µg/mL, respectively. As α-glucosidase inhibitory, the results of this study indicate can be used as a dietary supplement to manage hyperglycemia.
糖尿病是全球范围内主要的死亡原因。2019年，全球有4.63亿糖尿病患者，世界卫生组织预测到2030年这一数字将达到5.78亿。作为一种抗糖尿病药物，α-葡萄糖苷酶抑制剂是降低糖尿病患病率所采用的方法之一。糖尿病传统上被用作主要材料、药物、水果、观赏植物和木工原料来治疗。本研究旨在通过实验检测七种[植物名称未给出]对α-葡萄糖苷酶的抑制作用，分离活性物质并确定每个样品中它们的浓度。作为溶剂，乙醇用于浸渍提取物质。之后，提取物经过一系列分离技术，包括液-液萃取、真空液相色谱、柱色谱和制备薄层色谱（TLC）进行纯化和分离。使用TLC、紫外-可见分光光度法和核磁共振（NMR）光谱对化合物的结构进行了阐明。基于100和200μg/mL的浓度，[植物名称未给出]表现出最显著的抑制作用，其次是其他种类的[植物名称未给出]。与阳性对照阿卡波糖（IC55.39±0.67μg/mL）和槲皮苷（IC6.47±0.40μg/mL）相比，乙酸乙酯部分的活性最强（IC0.40±0.02μg/mL）。在[植物名称未给出]的乙酸乙酯部分中发现了扁蓄苷和4-O-甲基没食子酸，其IC值分别为17.05±0.75μg/mL和25.19±0.21μg/mL。作为α-葡萄糖苷酶抑制剂，本研究结果表明[植物名称未给出]可作为膳食补充剂用于控制高血糖。
