Clinic of Pediatric Respiratory Diseases and Tuberculosis, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, National Institute of Pediatric Tuberculosis and Respiratory Diseases, Dolny Smokovec, Slovak Republic, Department of Pathological Physiology, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, Slovak Republic.
Physiol Res. 2023 Jul 14;72(3):349-358. doi: 10.33549/physiolres.935063.
Bronchial asthma is the most common chronic respiratory disease of childhood. Cough is one of its defining symptoms. This study investigated the associations between selected inflammatory biomarkers and cough reflex sensitivity after capsaicin inhalation in children with mild and moderate well-controlled type 2 endotype asthma compared with non-asthmatic probands. Sensitivity to the cough reflex was measured by recording the cough response after capsaicin inhalation. The sandwich ELISA method was used to measure serum concentrations of the investigated potential inflammatory biomarkers (interleukin 13, interleukin 1beta, eosinophil-derived neurotoxin). The acquired data were statistically evaluated according to descriptive analyses for summarization and comparison between cough reflex sensitivity parameters and individual biomarker values in the observed and control groups modeled by a simple linear regression model. Statistical significance was defined as p<0.05. We showed a statistically significant association (p-value 0.03) between cough reflex sensitivity - C2 value (capsaicin concentration required for two cough responses) and interleukin 1beta serum concentrations in the asthma group compared with the control group of non-asthmatic children. Our results support the possibility of interleukin 1beta as a potential additive inflammatory biomarker used in clinical practice in children with asthma because of its correlation with the activity of the afferent nerve endings in the airways.
支气管哮喘是儿童最常见的慢性呼吸道疾病。咳嗽是其主要症状之一。本研究旨在比较轻中度 2 型表型哮喘患儿与非哮喘患儿之间，辣椒素吸入后咳嗽反射敏感性与选定炎症生物标志物之间的相关性。通过记录辣椒素吸入后的咳嗽反应来测量咳嗽反射敏感性。采用夹心 ELISA 法测定血清中潜在炎症生物标志物（白细胞介素 13、白细胞介素 1β、嗜酸性粒细胞衍生神经毒素）的浓度。根据描述性分析对所获得的数据进行了统计学评估，以总结和比较观察组和对照组之间的咳嗽反射敏感性参数和各个生物标志物值，采用简单线性回归模型进行建模。统计学意义定义为 p<0.05。与非哮喘儿童的对照组相比，哮喘组中咳嗽反射敏感性-C2 值（引起两次咳嗽反应所需的辣椒素浓度）与白细胞介素 1β血清浓度之间存在统计学显著相关性（p 值为 0.03）。我们的研究结果支持白细胞介素 1β作为一种潜在的附加炎症生物标志物在哮喘患儿临床实践中的应用的可能性，因为它与气道传入神经末梢的活性相关。
