Department of Physiology, Dokuz Eylul University Medical Faculty, Balcova, Izmir, Republic of Türkiye.
Physiol Res. 2023 Jul 14;72(3):383-392. doi: 10.33549/physiolres.935091.
Regular physical exercise is beneficial to the body. Acute exercise causes oxidant stress in many tissues including the liver by creating an unbalanced status between oxidant and antioxidant levels. Analgesic drugs are commonly consumed to reduce the pain after exercise. Acetaminophen (APAP), commonly used as an over-the-counter analgesic, can cause hepatotoxicity. The aim of this study was to investigate the effect and underlying mechanisms of APAP at subtoxic dose, which is given after the acute and exhaustive exercise on the rat livers. Male Wistar rats weighing 200-250 g were divided into 6 groups each consisting of 7 rats/group; Control, APAP (250 mg/kg, ip), Acute Exercise (AEx), Acute Exhaustive Exercise (AEEx), Acute Exercise and APAP (AEx+APAP) and Acute Exhaustive Exercise and APAP (AEEx+APAP) groups. Rats were exercised at moderate intensity or exhaustive on the treadmill and then received APAP. Tissue MDA levels were significantly increased in AEEx, AEx+APAP and AEEx+APAP groups compared with the control. There was no significant difference in GSH levels between groups. Tissue Sirtuin1 (Sirt1) levels of APAP, AEx and AEEx groups were significantly less than control. There was no significant difference between groups in VEGF levels. Liver damage score was significantly higher in all groups compared with control group. As a result, this study shows that subtoxic dose of APAP treatment alone or in combination with acute or exhaustive treadmill exercise can cause oxidative liver damage by affecting Sirt1 levels and without affecting VEGF levels.
定期进行体育锻炼对身体有益。剧烈运动通过在氧化剂和抗氧化剂水平之间造成不平衡状态，会使包括肝脏在内的许多组织产生氧化应激。人们通常会服用止痛药物来减轻运动后的疼痛。醋氨酚（APAP）常用作非处方止痛药，可导致肝毒性。本研究旨在探讨亚毒性剂量的 APAP 在急性和剧烈运动后对大鼠肝脏的影响及其潜在机制。雄性 Wistar 大鼠体重 200-250g，分为 6 组，每组 7 只；对照组、APAP（250mg/kg，ip）组、急性运动（AEx）组、剧烈运动（AEEx）组、急性运动加 APAP（AEx+APAP）组和剧烈运动加 APAP（AEEx+APAP）组。大鼠在跑步机上以中等强度或剧烈运动，然后给予 APAP。AEEx、AEx+APAP 和 AEEx+APAP 组的组织 MDA 水平明显高于对照组。各组 GSH 水平无显著差异。APAP、AEx 和 AEEx 组的组织 Sirtuin1（Sirt1）水平明显低于对照组。各组 VEGF 水平无显著差异。与对照组相比，所有组的肝损伤评分均显著升高。因此，本研究表明，亚毒性剂量的 APAP 单独或与急性或剧烈跑步机运动联合治疗可通过影响 Sirt1 水平而不影响 VEGF 水平导致氧化肝损伤。
