Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Methods Mol Biol. 2023;2695:309-315. doi: 10.1007/978-1-0716-3346-5_21.
Allografts rejection remains the most important reason causing allograft dysfunction in heart transplantation recipients. Currently, the golden standard for detecting graft rejection is endomyocardial biopsy (EMB). As a new noninvasive technique, liquid biopsy emerges along with the great developments of droplet-based digital PCR and the various optimizations of next-generation sequencing technologies, which is also cheaper than EMB. This review introduces several types of liquid biopsy and its application in heart transplantation.
同种异体移植物排斥仍然是导致心脏移植受者移植物功能障碍的最重要原因。目前，检测移植物排斥的金标准是心内膜心肌活检（EMB）。作为一种新的非侵入性技术，液体活检随着基于液滴的数字 PCR 的巨大发展和下一代测序技术的各种优化而出现，而且比 EMB 更便宜。本文介绍了几种液体活检及其在心脏移植中的应用。
