Department of Plant Pathology, Washington State University, Pullman, WA, USA.
Methods Mol Biol. 2023;2690:133-135. doi: 10.1007/978-1-0716-3327-4_13.
Forster resonance energy transfer (FRET) is an efficient method to visualize the protein-protein interaction in living cells. This technique is based on transfer of energy between two different fluorophores that are fused to two interacting proteins. In this chapter, we described the FRET assay to visualize the protein-protein interaction in plant cells.
Förster 共振能量转移（FRET）是一种在活细胞中可视化蛋白质-蛋白质相互作用的有效方法。该技术基于融合到两个相互作用的蛋白质中的两个不同荧光团之间的能量转移。在本章中，我们描述了用于可视化植物细胞中蛋白质-蛋白质相互作用的 FRET 测定法。
