Department of Gerontology and Metabolism, University Hospital and Medical Faculty in Hradec Kralove, Charles University in Prague, Czech Republic.
Department of Clinical Microbiology, University Hospital and Medical Faculty in Hradec Kralove, Charles University in Prague, Czech Republic.
Nutrition. 2023 Oct;114:112115. doi: 10.1016/j.nut.2023.112115. Epub 2023 Jun 4.
To evaluate the effectiveness of various taurolidine solutions in the prevention and treatment of catheter-related bloodstream infections (CRBSIs) caused by the entire spectrum of microbes in patients receiving parenteral nutrition in a shorter period of time.
The in vitro method was used to test for eradication of biofilm. Different locks were used: TauroSept (2%), TauroLock (1.35%), TauroLock half concentration, and 3.5% taurolidine and tested on Staphylococcus (S.) epidermidis, S. aureus, S. hominis, methicillin-resistant S. aureus (MRSA), Pseudomonas (P.) aeruginosa (PSAE), multidrug-resistant P. aeruginosa (MR PSAE), vancomycin-resistant enterococci, Klebsiella pneumoniae producing carbapenemase (KPC), Klebsiella pneumoniae producing extended-spectrum beta-lactamase (KLPN ESBL), Candida (C.) albicans, and C. glabrata. Broviac catheters were incubated for growth of each organism and then incubated in lock solutions. Colony forming units (CFUs) were then counted after 30 min, 60 min, and 120 min of incubation.
A statistically significant decrease in CFUs was observed after 30 min of taurolidine exposure for S. hominis, PSAE, KLPN ESBL, KLPN KPC, C. albicans, and C. glabrata; after 60 min of exposure for S. epidermidis, PSAE, MR PSAE, KLPN ESBL, KPC, C. albicans, and C. glabrata; and after 120 min of exposure for S. epidermidis, S. hominis, S. aureus, PSAE, MR PSAE, KLPN ESBL, KPC, C. albicans, C. glabrata.
The application of taurolidine is effective in the treatment of CRBSIs. Taurolidine proved to be more effective against Gram-negative microorganisms during a 30-min exposure. Using 0.675% taurolidine is still effective. To achieve the required antimicrobial effect, the catheter must be sanitized for at least 2 h.
评估不同牛磺罗定溶液在更短时间内预防和治疗接受肠外营养患者由全谱微生物引起的导管相关血流感染（CRBSI）的效果。
采用体外法检测生物膜清除率。使用不同的封管液：牛磺罗定（2%）、牛磺罗定（1.35%）、牛磺罗定半浓度、3.5%牛磺罗定，针对表皮葡萄球菌（S. epidermidis）、金黄色葡萄球菌（S. aureus）、人葡萄球菌（S. hominis）、耐甲氧西林金黄色葡萄球菌（MRSA）、铜绿假单胞菌（P. aeruginosa）（PSAE）、多重耐药铜绿假单胞菌（MRPSA）、万古霉素耐药肠球菌（VRE）、产碳青霉烯酶肺炎克雷伯菌（KPC）、产超广谱β-内酰胺酶肺炎克雷伯菌（KLPN ESBL）、白色念珠菌（C. albicans）和光滑念珠菌（C. glabrata）进行孵育。然后将每种生物的布鲁氏导管孵育并置于封管液中。孵育 30 分钟、60 分钟和 120 分钟后，计算集落形成单位（CFU）。
牛磺罗定暴露 30 分钟后，S. hominis、PSAE、KLPN ESBL、KLPN KPC、C. albicans 和 C. glabrata 的 CFU 显著减少；暴露 60 分钟后，S. epidermidis、PSAE、MR PSAE、KLPN ESBL、KPC、C. albicans 和 C. glabrata 的 CFU 显著减少；暴露 120 分钟后，S. epidermidis、S. hominis、S. aureus、PSAE、MR PSAE、KLPN ESBL、KPC、C. albicans 和 C. glabrata 的 CFU 显著减少。
牛磺罗定的应用在治疗 CRBSI 方面是有效的。牛磺罗定在 30 分钟暴露时间内对革兰氏阴性微生物更有效。使用 0.675%的牛磺罗定仍然有效。要达到所需的抗菌效果，导管必须消毒至少 2 小时。
