Department of Chemistry, Virginia Tech, 1040 Drillfiled Drive,171 Davidson Hall, Blacksburg, VA 24061, USA.
Perfetti and Perfetti, LLC, Winston-Salem, NC 27103, USA.
J Chromatogr Sci. 2024 May 31;62(5):492-497. doi: 10.1093/chromsci/bmad048.
An optimized method employing chiral supercritical fluid chromatography with diode array UV-VIS detection has been developed for the quantitative analysis of nicotine and nornicotine enantiomer distributions. The method parameters that were optimized included: column type (stationary phases, Chiralpak IG-3), column temperature (40°C), modifier types and concentration (isopropyl alcohol, 10%), additive types and concentrations (diethylamine, 0.2%), elution times (<6 min, flow rate 3 mL/min) and resolution factor (>1.2). These optimized conditions led to nicotine and nornicotine enantiomer detection limits of ~5 ng/μL with accompanying %RSD values of <2% from the analyses of commercially available nicotine-containing formulations.
建立了一种采用手性超临界流体色谱与二极管阵列紫外-可见检测相结合的优化方法，用于定量分析尼古丁和去甲烟碱对映体的分布。优化的方法参数包括：色谱柱类型（固定相，Chiralpak IG-3）、柱温（40°C）、改性剂类型和浓度（异丙醇，10%）、添加剂类型和浓度（二乙胺，0.2%）、洗脱时间（<6 min，流速 3 mL/min）和分辨率因子（>1.2）。在这些优化条件下，对市售含尼古丁制剂进行分析，尼古丁和去甲烟碱对映体的检测限约为 5 ng/μL，相应的 %RSD 值<2%。
