Department of Environmental Microbiology and Biotechnology, Nicolaus Copernicus University in Toruń, Lwowska 1, 87-100 Toruń, Poland.
Department of Environmental Microbiology and Biotechnology, Nicolaus Copernicus University in Toruń, Lwowska 1, 87-100 Toruń, Poland.
Microbiol Res. 2023 Oct;275:127448. doi: 10.1016/j.micres.2023.127448. Epub 2023 Jul 13.
Bacillus sp. is one of the best-studied plant growth-promoting rhizobacteria (PGPR). However, more detailed studies targeting its effect on the rhizosphere microbial community are required for improving management practices regarding its commercial application in the field. Our earlier study showed that PGPR Bacillus paralicheniformis 2R5 stimulated canola growth. Hence, this study aimed to assess the time-course impact of B. paralicheniformis 2R5 on bacterial and fungal community structure and diversity. The results showed that inoculation with B. paralicheniformis 2R5 initially significantly decreased the observed bacterial richness compared to the control, while after 44 days of treatment this alpha diversity metrics increased. A linear discriminant analysis effect size showed that B. paralicheniformis 2R5 altered the soil bacterial and fungal community structure by increasing the abundance of plants' beneficial microorganisms such as Nitrospira, Ramlibacter, Sphingomonas, Massilia, Terrimonas as well as Solicoccozyma, Schizothecium, Cyphellophora, Fusicolla, Humicola. B. paralicheniformis 2R5 seems to be a promising alternative to chemical pesticides and can be considered for practical application in the field. Its ability to alter the rhizosphere microbiome by increasing the diversity and composition of bacterial communities and increasing plants' beneficial groups of fungi, appears to be important in terms of improving canola development. However, further studies on these increased microbial taxa are necessary to confirm their function in promoting canola growth.
芽孢杆菌属是研究最多的植物促生根际细菌（PGPR）之一。然而，为了改善其在田间的商业应用的管理实践，需要针对其对根际微生物群落的影响进行更详细的研究。我们之前的研究表明，PGPR 地衣芽孢杆菌 2R5 刺激油菜生长。因此，本研究旨在评估 2R5 对地衣芽孢杆菌对细菌和真菌群落结构和多样性的时间进程影响。结果表明，与对照相比，接种 2R5 地衣芽孢杆菌初期显著降低了观察到的细菌丰富度，而在处理 44 天后，这些 α 多样性指标增加。线性判别分析效应大小表明，2R5 地衣芽孢杆菌通过增加植物有益微生物的丰度（如硝化螺旋菌、珊瑚菌、鞘氨醇单胞菌、马塞利亚菌、不动杆菌以及 Solicozyma、石蟹菌、Cyphellophora、Fusicolla、Humicola），改变了土壤细菌和真菌群落结构。2R5 地衣芽孢杆菌似乎是化学农药的一种有前途的替代品，可以考虑在田间实际应用。它通过增加细菌群落的多样性和组成以及增加植物有益真菌群来改变根际微生物组的能力，似乎在改善油菜发育方面很重要。然而，需要进一步研究这些增加的微生物类群，以确认它们在促进油菜生长方面的功能。
