Beneficial interactions between plants and bacteria are crucial in agricultural practices, as bacteria can improve soil fertility, promote plant growth, and protect plants from pathogens. This study aimed to molecularly identify and characterize soil bacterial isolates and evaluate their effect on the growth of maize ( L.), tomato ( L.), cucumber ( L.), and pepper ( L.) under greenhouse conditions. Plant growth parameters, including plant height, root length, and fresh (FW) and dry (DW) weights, were measured. Additionally, pigment extraction and element content analysis using leaves were performed. Among the isolates, the most effective strain in the greenhouse experiment was SCF6, which significantly enhanced plant height and fresh weight across all tested plants, with the greatest influence observed in maize plant height (439.42 ± 6.42 mm), fresh weight (14.07 ± 0.87 g plant FW), and dry weight (1.43 ± 0.17 g plant DW) compared to the control (364.67 ± 10.33 mm, 9.20 ± 1.16 g plant FW, and 0.92 ± 0.15 g plant DW, respectively). Other strains also demonstrated notable results, with SCF4, SCF8, and SCF9 showing the highest plant growth-promoting effects on pepper, tomato, and cucumber, respectively. SCF9 demonstrated strong antifungal activity against , with a percentage of mycelial growth inhibition (PGI) of 72.62 ± 2.06%, while SCF1 exhibited effects against (PGI = 69.57 ± 4.35%) and sp. (PGI = 63.11 ± 1.12%). The development and application of beneficial bacterial inoculants or their formulated products can contribute to environmentally friendly farming practices and global food security.