Song Yura, Fioramonti Marco, Bouvencourt Gaëlle, Dubois Christine, Blanpain Cédric, Van Keymeulen Alexandra
Laboratory of Stem Cells and Cancer, Université Libre de Bruxelles (ULB), Brussels, Belgium.
WELBIO, Université Libre de Bruxelles (ULB), Brussels, Belgium.
Heliyon. 2023 Jun 30;9(7):e17842. doi: 10.1016/j.heliyon.2023.e17842. eCollection 2023 Jul.
The mammary gland (MG) is composed of three main epithelial lineages, the basal cells (BC), the estrogen receptor (ER) positive luminal cells (ER+ LC), and the ER negative LC (ER- LC). Defining the cell identity of each lineage and how it is modulated throughout the different stages of life is important to understand how these cells function and communicate throughout life. Here, we used transgenic mice specifically labelling ER+ LC combined to cell surface markers to isolate with high purity the 3 distinct cell lineages of the mammary gland and defined their expression profiles and chromatin landscapes by performing bulk RNAseq and ATACseq of these isolated populations in puberty, adulthood and mid-pregnancy. Our analysis identified conserved genes, ligands and transcription factor (TF) associated with a specific lineage throughout life as well as genes, ligands and TFs specific for a particular stage of the MG. In summary, our study identified genes and TF network associated with the identity, function and cell-cell communication of the different epithelial lineages of the MG at different stages of life.
乳腺（MG）由三种主要的上皮谱系组成，即基底细胞（BC）、雌激素受体（ER）阳性的管腔细胞（ER+ LC）和ER阴性的管腔细胞（ER- LC）。明确每个谱系的细胞特性以及其在生命不同阶段是如何被调节的，对于理解这些细胞在整个生命过程中的功能和相互作用至关重要。在此，我们使用了特异性标记ER+ LC的转基因小鼠，并结合细胞表面标志物，以高纯度分离出乳腺的3种不同细胞谱系，并通过对青春期、成年期和妊娠中期这些分离群体进行大量RNA测序和转座酶可及染色质测序（ATACseq）来确定它们的表达谱和染色质景观。我们的分析确定了在整个生命过程中与特定谱系相关的保守基因、配体和转录因子（TF），以及乳腺特定阶段特有的基因、配体和TF。总之，我们的研究确定了与乳腺不同上皮谱系在生命不同阶段的特性、功能和细胞间通讯相关的基因和TF网络。
