Gao Jianpeng, Wang Hufei, Li Ming, Liu Zhongyang, Cheng Junyao, Liu Xiao, Liu Jianheng, Wang Xing, Zhang Licheng
Department of Orthopaedics, Chinese PLA General Hospital, 100039 Beijing, China.
Medical School of Chinese PLA, 100039 Beijing, China.
Int J Bioprint. 2023 Mar 16;9(5):754. doi: 10.18063/ijb.754. eCollection 2023.
Intramembranous ossification (IMO) and endochondral ossification (ECO) are two pathways of bone regeneration. The regeneration of most bone, such as limb bone, trunk bone, and skull base bone, mainly occurs in the form of endochondral ossification, which has also become one of the effective ways for bone tissue engineering. In this work, we prepared a well-structured and biocompatible methacrylated gelatin/polymethacrylic acid (GelMA/PMAA) hydrogel by digital light processing (DLP) printing technology, which could effectively chelate iron ions and continuously activate the hypoxia-inducible factor-1 alpha (HIF-1α) signaling pathway to promote the process of endochondral ossification and angiogenesis. The incorporation of PMAA endowed the hydrogel with remarkable viscoelasticity and high efficacy in chelation of iron ions, giving rise to the activation of HIF-1α signaling pathway, improving chondrogenic differentiation in the early stage, and facilitating vascularization in the later stage and bone remodeling. Therefore, the findings have significant implications on DLP printing technology of endochondral osteogenesis induced by the iron-chelating property of biological scaffold, which will provide an effective way in the development of novel bone regeneration.
膜内成骨（IMO）和软骨内成骨（ECO）是骨再生的两种途径。大多数骨骼的再生，如四肢骨、躯干骨和颅底骨，主要以软骨内成骨的形式发生，这也已成为骨组织工程的有效途径之一。在这项工作中，我们通过数字光处理（DLP）打印技术制备了一种结构良好且具有生物相容性的甲基丙烯酸化明胶/聚甲基丙烯酸（GelMA/PMAA）水凝胶，该水凝胶能够有效螯合铁离子并持续激活缺氧诱导因子-1α（HIF-1α）信号通路，以促进软骨内成骨和血管生成过程。PMAA的加入赋予了水凝胶显著的粘弹性和高效的铁离子螯合能力，从而激活HIF-1α信号通路，改善早期软骨生成分化，并在后期促进血管化和骨重塑。因此，这些发现对生物支架铁螯合特性诱导软骨内成骨的DLP打印技术具有重要意义，这将为新型骨再生的发展提供有效途径。
