National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hubei Key Laboratory of Plant Pathology, The Center for Crop Nanobiotechnology, Huazhong Agricultural University, Wuhan, China.
College of Science, Huazhong Agricultural University, Wuhan, China.
J Pineal Res. 2023 Sep;75(2):e12896. doi: 10.1111/jpi.12896. Epub 2023 Jul 17.
Melatonina natural harmless molecule-displays versatile roles in human health and crop disease control such as for rice blast. Rice blast, caused by the filamentous fungus Magnaporthe oryzae, is one devastating disease of rice. Application of fungicides is one of the major measures in the control of various crop diseases. However, fungicide resistance in the pathogen and relevant environmental pollution are becoming serious problems. By screening for possible synergistic combinations, here, we discovered an eco-friendly combination for rice blast control, melatonin, and the fungicide isoprothiolane. These compounds together exhibited significant synergistic inhibitory effects on vegetative growth, conidial germination, appressorium formation, penetration, and plant infection by M. oryzae. The combination of melatonin and isoprothiolane reduced the effective concentration of isoprothiolane by over 10-fold as well as residual levels of isoprothiolane. Transcriptomics and lipidomics revealed that melatonin and isoprothiolane synergistically interfered with lipid metabolism by regulating many common targets, including the predicted isocitrate lyase-encoding gene MoICL1. Furthermore, using different techniques, we show that melatonin and isoprothiolane interact with MoIcl1. This study demonstrates that melatonin and isoprothiolane function synergistically and can be used to reduce the dosage and residual level of isoprothiolane, potentially contributing to the environment-friendly and sustainable control of crop diseases.
褪黑素天然无害分子-在人类健康和作物病害控制中发挥多种作用，如稻瘟病。稻瘟病是由丝状真菌稻瘟病菌引起的，是水稻的一种毁灭性病害。应用杀菌剂是防治各种作物病害的主要措施之一。然而，病原菌的抗药性和相关的环境污染问题日益严重。通过筛选可能的协同组合，在这里，我们发现了一种用于稻瘟病控制的环保组合，褪黑素和杀菌剂异噻菌胺。这些化合物联合使用对稻瘟病菌的营养生长、孢子萌发、附着胞形成、穿透和植物感染具有显著的协同抑制作用。褪黑素和异噻菌胺的组合使异噻菌胺的有效浓度降低了 10 倍以上，同时降低了异噻菌胺的残留水平。转录组学和脂质组学揭示，褪黑素和异噻菌胺通过调节许多共同的靶标，包括预测的异柠檬酸裂解酶编码基因 MoICL1，协同干扰脂质代谢。此外，我们还使用不同的技术表明褪黑素和异噻菌胺与 MoIcl1 相互作用。这项研究表明，褪黑素和异噻菌胺具有协同作用，可以降低异噻菌胺的用量和残留水平，为作物病害的环保和可持续控制做出贡献。
