Institute of Sustainable Processes, University of Valladolid; Department of Chemical Engineering and Environmental Technology, University of Valladolid; Department of Public Health Microbiology, National Laboratory of Health, Environment and Food;
ICBAS - School of Medicine and Biomedical Sciences, Porto University; Epidemiology Research Unit (EPIUnit), Instituto de Saúde Pública da Universidade do Porto; Laboratório para a Investigação Integrativa e Translacional em Saúde Populacional (ITR);
J Vis Exp. 2023 Jun 30(196). doi: 10.3791/65053.
Wastewater-based epidemiology has emerged as a promising and efficacious surveillance system for SARS-CoV-2 and other infectious diseases in many nations. The process typically involves wastewater concentration, nucleic acid extraction, amplification of selected genomic segments, and detection and quantification of the amplified genomic segment. This methodology can similarly be leveraged to detect and quantify infectious agents, such as SARS-CoV-2, in air samples. Initially, SARS-CoV-2 was presumed to spread primarily through close personal contact with droplets generated by an infected individual while speaking, sneezing, coughing, singing, or breathing. However, a growing number of studies have reported the presence of SARS-CoV-2 RNA in the air of healthcare facilities, establishing airborne transmission as a viable route for the virus. This study presents a composite of established protocols to facilitate environmental detection, quantification, and sequencing of viruses from both wastewater and air samples.
污水流行病学已成为许多国家监测 SARS-CoV-2 和其他传染病的一种有前途和有效的方法。该过程通常包括污水浓缩、核酸提取、选定基因组片段的扩增以及扩增基因组片段的检测和定量。这种方法也可以用于检测和定量空气中的传染性病原体，如 SARS-CoV-2。最初，人们认为 SARS-CoV-2 主要通过与感染者说话、打喷嚏、咳嗽、唱歌或呼吸时产生的飞沫密切接触传播。然而，越来越多的研究报告称，在医疗机构的空气中存在 SARS-CoV-2 RNA，这表明空气传播是病毒传播的一种可行途径。本研究综合了现有的方案，以促进从污水和空气样本中检测、定量和测序病毒。
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