Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine.
Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine;
J Vis Exp. 2023 Jun 30(196). doi: 10.3791/65451.
Clinical trials suggest that Tuina manipulation is effective in treating knee osteoarthritis (KOA), while further studies are required to discover its mechanism. Therefore, the manipulation of animal models of knee osteoarthritis is critical. This protocol provides a standard process for Tuina manipulation on KOA rats and a preliminary exploration of the mechanism of Tuina for KOA. The press and kneading manipulation method (a kind of Tuina manipulation that refers to pressing and kneading the specific area of the body surface) is applied on 5 acupoints around the knee joint of rats. The force and frequency of the manipulation were standardized by finger pressure recordings, and the position of the rat during manipulation is described in detail in the protocol. The effect of manipulation can be measured by pain behavior tests and microscopic findings in synovial and cartilage. KOA rats showed significant improvement in pain behavior. The synovial tissue inflammatory infiltration was reduced in the Tuina group, and the expression of tumor necrosis factor (TNF)-α was significantly lower. Compared to the control group, chondrocyte apoptosis was less in the Tuina group. This study provides a standardized protocol for Tuina manipulation on KOA rats and preliminary proof that the therapeutic effects of Tuina may be related to reducing synovial inflammation and delayed chondrocyte apoptosis.
临床试验表明推拿手法治疗膝骨关节炎（KOA）有效，但其作用机制尚需进一步研究。因此，对膝骨关节炎动物模型进行推拿手法操作至关重要。本方案提供了一种对 KOA 大鼠进行推拿手法操作的标准流程，并初步探讨了推拿治疗 KOA 的作用机制。采用按压揉捏法（一种推拿手法，是指按压揉捏身体特定部位）对大鼠膝关节周围的 5 个穴位进行操作。通过指压记录标准化了手法的力度和频率，并在方案中详细描述了大鼠在操作过程中的位置。通过疼痛行为测试和滑膜及软骨的显微镜检查来评估手法的效果。推拿组大鼠的疼痛行为明显改善。滑膜组织的炎症浸润减少，肿瘤坏死因子（TNF）-α 的表达明显降低。与对照组相比，推拿组软骨细胞凋亡减少。本研究为 KOA 大鼠的推拿手法操作提供了标准化方案，并初步证明推拿的治疗效果可能与减轻滑膜炎症和延迟软骨细胞凋亡有关。
