Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine.
Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine.
J Vis Exp. 2024 Jan 12(203). doi: 10.3791/65770.
Knee osteoarthritis (KOA), a common degenerative joint disorder, is characterized by chronic pain and disability, which can progress to irreparable structural damage of the joint. Investigations into the link between articular cartilage, muscles, synovium, and other tissues surrounding the knee joint in KOA are of great importance. Currently, managing KOA includes lifestyle modifications, exercise, medication, and surgical interventions; however, the elucidation of the intricate mechanisms underlying KOA-related pain is still lacking. Consequently, KOA pain remains a key clinical challenge and a therapeutic priority. Tuina has been found to have a regulatory effect on the motor, immune, and endocrine systems, prompting the exploration of whether Tuina could alleviate KOA symptoms, caused by the upregulation of inflammatory factors, and further, if the inflammatory factors in skeletal muscle can augment the progression of KOA. We randomized 32 male Sprague Dawley (SD) rats (180-220 g) into four groups of eight animals each: antiPD-L1+Tuina (group A), model (group B), Tuina (group C), and sham surgery (group D). For groups A, B, and C, we injected 25 µL of sodium monoiodoacetate (MIA) solution (4 mg MIA diluted in 25 µL of sterile saline solution) into the right knee joint cavity, and for group D, the same amount of sterile physiological saline was injected. All the groups were evaluated using the least to most stressful tests (paw mechanical withdrawal threshold, paw withdrawal thermal latency, swelling of the right knee joint, Lequesne MG score, skin temperature) before injection and 2, 9, and 16 days after injection.
膝骨关节炎（KOA）是一种常见的退行性关节疾病，其特征为慢性疼痛和功能障碍，病情可进展至不可逆转的关节结构破坏。研究 KOA 关节软骨、肌肉、滑膜及膝关节周围其他组织之间的联系具有重要意义。目前，KOA 的管理包括生活方式改变、运动、药物和手术干预；然而，阐明 KOA 相关疼痛的复杂机制仍存在不足。因此，KOA 疼痛仍然是一个关键的临床挑战和治疗重点。推拿对运动、免疫和内分泌系统具有调节作用，这促使人们探索推拿是否可以缓解 KOA 症状，减轻炎症因子的上调，并进一步研究骨骼肌中的炎症因子是否会加剧 KOA 的进展。我们将 32 只雄性 Sprague Dawley（SD）大鼠（180-220 g）随机分为四组，每组 8 只：抗 PD-L1+推拿（A 组）、模型（B 组）、推拿（C 组）和假手术（D 组）。对于 A、B 和 C 组，我们将 25 µL 浓度为 4 mg/ml 的单碘乙酸钠（MIA）溶液（MIA 溶解于 25 µL 无菌生理盐水溶液中）注入右侧膝关节腔，而 D 组则注入等量的无菌生理盐水。所有组在注射前及注射后第 2、9 和 16 天，采用从最轻微到最具压力的测试（足底机械撤回阈值、足底撤回热潜伏期、右膝关节肿胀、Lequesne MG 评分、皮肤温度）进行评估。
