Department of Clinical Pathology, Faculty of Medicine, Universitas Indonesia/Cipto Mangunkusumo General Hospital, Jakarta, Indonesia.
Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia/Cipto Mangunkusumo General Hospital, Jakarta, Indonesia.
PLoS One. 2023 Jul 17;18(7):e0287399. doi: 10.1371/journal.pone.0287399. eCollection 2023.
This research is a preliminary study to observe the high-risk HPV infection profile among asymptomatic women, as a basis for developing Indonesian-specific reagents and implementing a national vaccination program.
The research subjects were female employees/families of BUMN (state-owned enterprises) who underwent annual routine medical check-up. The research sample was a cervical swab which was examined using the Cobas® 6800 platform for HPV identification and INNO Lipa (Fujirebio) kit for identification of the genotype.
Out of 858 cervical swab samples collected in this study, 31 samples were excluded as they had invalid results from the initial examination, so the remaining 827 samples continued this study protocol. Of those samples, 69 (8%) samples gave positive results, giving an overall HPV prevalence of 8%. Among HPV-positive samples, HPV 52 was the most common genotype (N = 15, 21.7%) found as both single-infection and multiple infections. The median age of subjects was 38 years. There were nine samples (13%) with multiple infections of two or more genotypes and seven samples (10.1%) with no specific genotype identified.
HPV prevalence was 8%, with HPV 52 being the most common high-risk type, making it a necessity to develop a diagnostic kit and vaccine for national vaccination program that is specific for Indonesian population which includes this genotype.
本研究旨在观察无症状女性中高危型 HPV 感染情况，为开发印尼特有的试剂和实施国家疫苗接种计划提供依据。
研究对象为接受年度常规体检的国有企业（BUMN）女性员工/家属。研究样本为宫颈拭子，采用 Cobas® 6800 平台进行 HPV 鉴定，以及 INNO Lipa（Fujirebio）试剂盒进行基因型鉴定。
在本研究中，共采集了 858 例宫颈拭子样本，其中 31 例由于初步检查结果无效而被排除，因此剩余的 827 例继续进行研究方案。在这些样本中，有 69 例（8%）呈阳性，HPV 总阳性率为 8%。在 HPV 阳性样本中，HPV 52 是最常见的基因型（N=15，21.7%），包括单一感染和多重感染。受试者的中位年龄为 38 岁。有 9 例（13%）为两种或两种以上基因型的多重感染，7 例（10.1%）未鉴定出特定基因型。
HPV 阳性率为 8%，HPV 52 是最常见的高危型，因此有必要开发针对包括该基因型在内的印尼人群的诊断试剂盒和疫苗，用于国家疫苗接种计划。
