Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, Institute of Medical Biophysics, Mala Hora 4, 03601 Martin, Slovakia.
Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, Institute of Medical Biophysics, Mala Hora 4, 03601 Martin, Slovakia.
Respir Physiol Neurobiol. 2023 Sep;315:104115. doi: 10.1016/j.resp.2023.104115. Epub 2023 Jul 17.
Bicuculline and saclofen were microinjected into the rostral (rNTS) and caudal nucleus of the solitary tract (cNTS) in 17 anesthetized cats. Electromyograms (EMGs) of the diaphragm (DIA) and abdominal muscles (ABD), esophageal pressures (EP), and blood pressure were recorded and analyzed. Bilateral microinjections of 1 mM bicuculline in the rNTS significantly reduced the number of coughs (CN), amplitudes of DIA and ABD EMG, inspiratory and expiratory EP, and prolonged the duration of the cough expiratory phase (CTE) as well as the total cough cycle duration (CTtot). Bilateral microinjections of 2 mM saclofen reduced only cough expiratory efforts. Bilateral microinjection of bicuculline in the cNTS significantly reduced CN and amplitudes of ABD EMG and elongated CTE and CTtot. Bilateral microinjections of saclofen in cNTS had no significant effect on analyzed cough parameters. Our results confirm a different GABAergic inhibitory system in the rNTS and cNTS acting on mechanically induced cough in cats.
在 17 只麻醉猫中，将比枯枯林和 saclofen 分别微注射到孤束核的头端（rNTS）和尾端（cNTS）。记录和分析膈（DIA）和腹肌（ABD）的肌电图（EMG）、食管压力（EP）和血压。双侧 rNTS 注射 1mM 比枯枯林可显著减少咳嗽次数（CN）、DIA 和 ABD EMG 幅度、吸气和呼气 EP 以及延长咳嗽呼气期持续时间（CTE）和总咳嗽周期持续时间（CTtot）。双侧 2mM saclofen 注射仅减少咳嗽呼气努力。双侧 rNTS 注射比枯枯林可显著减少 CN 和 ABD EMG 幅度，并延长 CTE 和 CTtot。双侧 cNTS 注射 saclofen 对分析的咳嗽参数没有显著影响。我们的结果证实，rNTS 和 cNTS 中的 GABA 能抑制系统在猫的机械性诱发咳嗽中作用不同。
