Suppr超能文献

RasGEF MoCdc25 调控水稻稻瘟病菌的营养生长、产孢和附着胞介导的侵染。

The RasGEF MoCdc25 regulates vegetative growth, conidiation and appressorium-mediated infection in the rice blast fungus Magnaporthe oryzae.

机构信息

State Key Laboratory of Rice Biology and Breeding & Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.

State Key Laboratory of Rice Biology and Breeding & Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.

出版信息

Fungal Genet Biol. 2023 Oct;168:103825. doi: 10.1016/j.fgb.2023.103825. Epub 2023 Jul 17.

Abstract

Ras guanine nucleotide exchange factors (RasGEFs) can trigger Ras GTPase activities and play important roles in controlling various cellular processes in eukaryotes. Recently, it has been exhibited that RasGEF Cdc25 regulates morphological differentiation and pathogenicity in several plant pathogenic fungi. However, the role of RasGEFs in Magnaporthe oryzae is largely unknown. In this study, we identified and functionally characterized a RasGEF gene MoCDC25 in M. oryzae, which is orthologous to Saccharomyces cerevisiae CDC25. Targeted gene deletion mutants (ΔMocdc25) were completely nonpathogenic and were severely impaired in hyphal growth, conidiation and appressorium formation. The mutants exhibited highly sensitive response to osmotic, cell wall integrity or oxidative stresses. MoCdc25 physically interacts with the MAPK scaffold Mst50 and the putative Cdc42GEF MoScd1 in yeast two-hybrid assays. Moreover, we found that MoCdc25 was involved in regulating the phosphorylation of the MAP kinases (Pmk1, Mps1, and Osm1). In addition, the intracellular cAMP content in hyphae of the ΔMocdc25 mutants was significantly reduced compared to the parent strain Ku80 and the defect of appressorium formation of the mutants could be partially restored by the supplement of exogenous cAMP. Taken together, we conclude that the RasGEF MoCdc25 regulates vegetative growth, conidiation, appressorium formation and pathogenicity via MAPK and cAMP response pathways in M. oryzae.

摘要

Ras 鸟嘌呤核苷酸交换因子(RasGEFs)可以触发 Ras GTP 酶的活性,并在真核生物中控制各种细胞过程中发挥重要作用。最近,已经表明 RasGEF Cdc25 调节几种植物病原真菌的形态分化和致病性。然而,RasGEFs 在稻瘟病菌中的作用在很大程度上是未知的。在本研究中,我们鉴定并功能表征了稻瘟病菌中的 RasGEF 基因 MoCDC25,它与酿酒酵母 CDC25 同源。靶向基因缺失突变体(ΔMocdc25)完全丧失了致病性,并且在菌丝生长、分生孢子形成和附着胞形成方面严重受损。突变体对渗透、细胞壁完整性或氧化应激表现出高度敏感的反应。MoCdc25 在酵母双杂交实验中与 MAPK 支架 Mst50 和假定的 Cdc42GEF MoScd1 发生物理相互作用。此外,我们发现 MoCdc25 参与调节 MAP 激酶(Pmk1、Mps1 和 Osm1)的磷酸化。此外,与亲本菌株 Ku80 相比,ΔMocdc25 突变体中的菌丝内 cAMP 含量显著降低,并且通过添加外源性 cAMP 可以部分恢复突变体附着胞形成的缺陷。总之,我们得出结论,RasGEF MoCdc25 通过 MAPK 和 cAMP 反应途径调节稻瘟病菌的营养生长、分生孢子形成、附着胞形成和致病性。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验