State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-products, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Central Laboratory, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Environ Microbiol. 2022 Mar;24(3):1653-1671. doi: 10.1111/1462-2920.15949. Epub 2022 Mar 7.
The development and pathogenicity of the fungus Magnaporthe oryzae, the causal agent of destructive rice blast disease, require it to perceive external environmental signals. Opy2, an overproduction-induced pheromone-resistant protein 2, is a crucial protein for sensing external signals in Saccharomyces cerevisiae. However, the biological functions of the homologue of Opy2 in M. oryzae are unclear. In this study, we identified that MoOPY2 is involved in fungal development, pathogenicity, and autophagy in M. oryzae. Deletion of MoOPY2 resulted in pleiotropic defects in hyphal growth, conidiation, germ tube extension, appressorium formation, appressorium turgor generation, and invasive growth, therefore leading to attenuated pathogenicity. Furthermore, MoOpy2 participates in the Osm1 MAPK pathway and the Mps1 MAPK pathway by interacting with the adaptor protein Mst50. The interaction sites of Mst50 and MoOpy2 colocalized with the autophagic marker protein MoAtg8 in the preautophagosomal structure sites (PAS). Notably, the ΔMoopy2 mutant caused cumulative MoAtg8 lipidation and rapid GFP-MoAtg8 degradation in response to nitrogen starvation, showing that MoOpy2 is involved in the negative regulation of autophagy activity. Taken together, our study revealed that MoOpy2 of M. oryzae plays an essential role in the orchestration of fungal development, appressorium penetration, autophagy and pathogenesis.
稻瘟病菌 Magnaporthe oryzae 的发育和致病性需要其感知外部环境信号。Opy2 是一种过量产生诱导的信息素抗性蛋白 2，是酿酒酵母中感知外部信号的关键蛋白。然而，稻瘟病菌中 Opy2 同源物的生物学功能尚不清楚。在本研究中，我们鉴定出 MoOPY2 参与了稻瘟病菌的发育、致病性和自噬。MoOPY2 的缺失导致菌丝生长、分生孢子形成、芽管延伸、附着胞形成、附着胞膨压产生和侵袭生长的多效缺陷，从而导致致病性减弱。此外，MoOpy2 通过与衔接蛋白 Mst50 相互作用参与 Osm1 MAPK 途径和 Mps1 MAPK 途径。Mst50 和 MoOpy2 的相互作用位点与自噬标记蛋白 MoAtg8 在 preautophagosomal structure sites (PAS) 中共定位。值得注意的是，ΔMoopy2 突变体在氮饥饿时导致累积的 MoAtg8 脂质化和 GFP-MoAtg8 的快速降解，表明 MoOpy2 参与了自噬活性的负调控。总之，我们的研究表明，稻瘟病菌的 MoOpy2 在真菌发育、附着胞穿透、自噬和致病性的协调中发挥着重要作用。
