Reeves Lawrence E, Burkett-Cadena Nathan D
University of Florida, Institute of Food and Agricultural Sciences, Department of Entomology and Nematology, Florida Medical Entomology Laboratory, Vero Beach, Florida 32962, USA
University of Florida, Institute of Food and Agricultural Sciences, Department of Entomology and Nematology, Florida Medical Entomology Laboratory, Vero Beach, Florida 32962, USA.
Cold Spring Harb Protoc. 2024 Oct 1;2024(10):pdb.top107706. doi: 10.1101/pdb.top107706.
The host associations of mosquitoes vary by species, with some species being relative generalists, whereas others specialize, to varying extents, on a particular subset of the available host community. These host associations are driving factors in transmission dynamics of mosquito-vectored pathogens. For this reason, characterizing the host associations of mosquito species is critical for understanding the epidemiology of mosquito-vectored pathogens. Diverse methods have been used to associate mosquito species with their hosts. These typically include collecting mosquitoes that bite a restrained host (bait) or collecting wild blood-engorged mosquitoes and matching their blood meal to reference samples (blood meal analysis). Blood meal analysis refers to a collection of molecular techniques for determining the taxonomic identity of the source of a mosquito blood meal using cytological, serological, or DNA-based characteristics of the blood meal. Blood meal analyses that are based on DNA markers have advantages over cytological and serological methods and are effective for determining species-level identities of hosts from a broad range of potential host taxa. Here, we discuss effective techniques for analyzing blood meals.
蚊子与宿主的关联因物种而异，有些物种相对具有普遍性，而其他物种则在不同程度上专门针对可用宿主群落中的特定子集。这些宿主关联是蚊媒病原体传播动态的驱动因素。因此，表征蚊子物种的宿主关联对于理解蚊媒病原体的流行病学至关重要。已使用多种方法将蚊子物种与其宿主关联起来。这些方法通常包括收集叮咬受限宿主（诱饵）的蚊子，或收集野外饱腹血的蚊子，并将它们的血餐与参考样本进行匹配（血餐分析）。血餐分析是指一系列分子技术，用于利用血餐的细胞学、血清学或基于DNA的特征来确定蚊子血餐来源的分类身份。基于DNA标记的血餐分析比细胞学和血清学方法具有优势，并且对于确定来自广泛潜在宿主类群的宿主物种水平身份有效。在这里，我们讨论分析血餐的有效技术。
