Gao Luyao, Cao Jiajian, Gong Siyu, Hao Ning, Du Yalin, Wang Chunhua, Wu Tao
College of Horticulture, Hunan Agricultural University, Changsha, 410128, China.
Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops (vegetables, tea, etc.), Ministry of Agriculture and Rural Affairs of China, Changsha, 410128, China.
Plant J. 2023 Oct;116(2):524-540. doi: 10.1111/tpj.16389. Epub 2023 Jul 26.
To improve our understanding of the mechanism underlying cucumber glossiness regulation, a novel cucumber mutant with a glossy peel (Csgp) was identified. MutMap, genotyping, and gene editing results demonstrated that CsSEC23, which is the core component of COPII vesicles, mediates the glossiness of cucumber fruit peel. CsSEC23 is functionally conserved and located in the Golgi and endoplasmic reticulum. CsSEC23 could interact with CsSEC31, but this interaction was absent in the Csgp mutant, which decreased the efficiency of COPII vesicle transportation. Genes related to wax and cutin transport were upregulated in the Csgp mutant, and the cuticle structure of the Csgp-mutant peel became thinner. Moreover, the wax and cutin contents were also changed due to CsSEC23 mutation. Taken together, the results obtained from this study revealed that CsSEC23 mediates cucumber glossiness, and this mediating might be affected by COPII vesicle transportation.
为了提高我们对黄瓜光泽度调控机制的理解，鉴定出了一种具有光滑果皮的新型黄瓜突变体（Csgp）。MutMap、基因分型和基因编辑结果表明，作为COPII囊泡核心成分的CsSEC23介导了黄瓜果实表皮的光泽度。CsSEC23在功能上是保守的，位于高尔基体和内质网中。CsSEC23可以与CsSEC31相互作用，但这种相互作用在Csgp突变体中不存在，这降低了COPII囊泡运输的效率。与蜡质和角质运输相关的基因在Csgp突变体中上调，Csgp突变体果皮的角质层结构变薄。此外，由于CsSEC23突变，蜡质和角质含量也发生了变化。综上所述，本研究结果表明CsSEC23介导黄瓜光泽度，且这种介导作用可能受COPII囊泡运输的影响。
