Wray W, Wray V P
J Histochem Cytochem. 1979 Jan;27(1):454-7. doi: 10.1177/27.1.374609.
The proteins on metaphase chromosomes theoretically may be distributed ubiquitously throughout the karyotype, may be present uniquely on individual chromosomes or classes of chromosomes, or may exist in any combination of the above. Separation of chromosomes according to size using sucrose velocity gradients in high capacity zonal centrifuge rotors allows sufficient fractionation of the genome to indicate the distribution of proteins within the karyotype. Flow cytometric analysis and direct microscopic analysis were used to evaluate qualitatively the types of chromosomes present in the fractions obtained. This report is the first quantitative evidence that some of the chromosomal proteins are not distributed ubiquitously on all of the chromosomes of the karyotype.
中期染色体上的蛋白质理论上可能普遍分布于整个核型中，可能仅存在于个别染色体或染色体类别上，或者可能以上述任何组合形式存在。在大容量区带离心机转子中使用蔗糖速度梯度按大小分离染色体，可对基因组进行充分分级分离，以表明蛋白质在核型中的分布情况。采用流式细胞术分析和直接显微镜分析对所获级分中存在的染色体类型进行定性评估。本报告首次提供了定量证据，证明某些染色体蛋白质并非普遍分布于核型的所有染色体上。
