Bell E, Levinstone D, Sher S, Marek L, Merrill C, Young I, Eden M
J Histochem Cytochem. 1979 Jan;27(1):458-62. doi: 10.1177/27.1.374610.
We have developed an interactive computer system for analysing cell lineage data. It can be utilized in studies of cell motility, cell division, cell differentiation, and cell aging. It has enabled us to document the heterogeneity of human foreskin fibroblasts in culture and to propose that loss of proliferative potential may mean that cells enter a state of differentiation which makes them unable to respond to mitotic stimulation. Our method, which enables us to apply immunological and cytochemical probes after recording the history of a cell lineage, should allow us to define precisely features which uniquely distinguish cycling from noncycling cells on an individual cell basis.
我们开发了一种用于分析细胞谱系数据的交互式计算机系统。它可用于细胞运动、细胞分裂、细胞分化和细胞衰老的研究。它使我们能够记录培养的人包皮成纤维细胞的异质性，并提出增殖潜能的丧失可能意味着细胞进入一种分化状态，使它们无法对有丝分裂刺激作出反应。我们的方法能够在记录细胞谱系历史后应用免疫和细胞化学探针，这应该使我们能够在单个细胞的基础上精确地定义区分循环细胞和非循环细胞的独特特征。
