Bell E, Marek L F, Levinstone D S, Merrill C, Sher S, Young I T, Eden M
Science. 1978 Dec 15;202(4373):1158-63. doi: 10.1126/science.725592.
We have examined the hypothesis that diploid cells grown in vitro age, and propose that only proliferative potential and not life-span is telescoped. We suggest that explanted or transplanted diploid cells are driven to divide by the process of subculturing in vitro or in vivo and, in response to this pressure, also complete their differentiation and become refractory to further mitotic stimulation. We conclude that differentiation rather than "mortality" distinguishes diploid from transformed cells and that the former may not age in vitro, but are lost because culture methods are selective for cycling cells.
我们检验了体外培养的二倍体细胞会衰老这一假说，并提出只有增殖潜能而非寿命被缩短。我们认为，体外或体内传代培养的过程驱使外植或移植的二倍体细胞进行分裂，并且，作为对这种压力的反应，它们也完成分化，不再对进一步的有丝分裂刺激产生反应。我们得出结论，分化而非“死亡”区分了二倍体细胞和转化细胞，前者在体外可能不会衰老，而是因为培养方法对处于增殖周期的细胞具有选择性而消失。
