Bennett D E, Mayall B H
J Histochem Cytochem. 1979 Jan;27(1):579-83. doi: 10.1177/27.1.374624.
An interactive computer program, SWELL, displayss and analyzes bivariate distributions generated by flow cytometers. SWELL is modular with options available via a menu, is written in Fortran, and utilizes a video color display system. Data are accumulated as a bivariate distribution that is transferred to the computer as a 64 x 64 matrix. For ease of visualization, matrices are displayed in pseudocolor. The distribution values are broken into eight ranges and each range is represented by a color. Each element of the matrix is then displayed in its assigned color. To allow pooling and comparison, distributions are aligned, edited, and standardized. Unknown samples are pooled or analyzed singly and compared to the normal pool by subtraction. Differences are displayed as pseudocolor matrices of sign, magnitude, or statistical magnitude in units of standard deviation. This latter display, scaled to tolerance limits, readily reveals regions of significant difference between normal and abnormal samples. Counts within such regions can be compared to diagnose samples automatically.
一个交互式计算机程序SWELL，用于显示和分析流式细胞仪生成的双变量分布。SWELL是模块化的，可通过菜单选择选项，用Fortran编写，并使用视频彩色显示系统。数据以双变量分布的形式积累，并作为一个64×64矩阵传输到计算机。为便于可视化，矩阵以伪彩色显示。分布值被分成八个范围，每个范围由一种颜色表示。然后矩阵的每个元素以其指定的颜色显示。为了进行合并和比较，对分布进行对齐、编辑和标准化。未知样本可以单独合并或分析，并通过减法与正常样本库进行比较。差异以符号、大小或标准差单位的统计大小的伪彩色矩阵显示。后一种显示按公差极限进行缩放，能很容易地揭示正常样本和异常样本之间的显著差异区域。可以比较这些区域内的计数以自动诊断样本。
