Härtel W, Betz J W, von Sengbusch G, Bader P
J Histochem Cytochem. 1979 Jan;27(1):311-4. doi: 10.1177/27.1.374593.
As a method for the preselection of alarms in gynecological cell samples, the Battelle Cytophotometry Research Group uses the slit-scan technique to obtain various cell parameters, such as the N/C ratio and the relative DNA content, from fluorescently stained cells, which are aligned one-dimensionally in the tape system designed at Battelle. The system developed at Battelle Institute analyzes all signals that exceed the background noise. As the first step in processing the slit-scan data, several threshold levels permit the separation of various artifacts. In subsequent steps, the nuclear peak is recognized, the nuclear boundaries are calculated, and seven cell parameters are determined. For the alarm detection at present only one parameter, DNA fluorescence, is used for these determinations. Visual assignment of these data to definite objects on the tape makes it possible to obtain frequency distributions of: (a) all recorded objects within the sample on the tape; (b) all signals that are classified as cells; and (c) all types of objects that preferentially cause alarms.
作为一种对妇科细胞样本中的警报进行预选的方法，巴特尔细胞光度法研究小组采用狭缝扫描技术从荧光染色细胞中获取各种细胞参数，如核质比和相对DNA含量，这些细胞在巴特尔设计的纸带系统中呈一维排列。巴特尔研究所开发的系统会分析所有超过背景噪声的信号。作为处理狭缝扫描数据的第一步，几个阈值水平可用于分离各种伪像。在后续步骤中，识别核峰，计算核边界，并确定七个细胞参数。目前在警报检测中，仅使用一个参数（DNA荧光）进行这些测定。将这些数据直观地分配到纸带上的特定对象上，就可以获得以下各项的频率分布：(a)纸带上样本内所有记录的对象；(b)所有被分类为细胞的信号；以及(c)所有优先引发警报的对象类型。
