Bogomolova N N, Koptyaeva I B, Giraudon P, Wild T F
J Gen Virol. 1986 Sep;67 ( Pt 9):1979-85. doi: 10.1099/0022-1317-67-9-1979.
The synthesis of intracellular measles virus proteins in persistently infected human cell cultures was studied. The virus-induced proteins were analysed after radioimmunoprecipitation by one- and two-dimensional polyacrylamide gel electrophoresis. The measles virus-induced nucleoprotein (NP) synthesized in persistently infected cells had a reduced binding capacity with measles virus antibodies (human convalescent serum) compared to the NP protein induced by the virus used to initiate the infection. In contrast, monospecific rabbit serum prepared against the original virus NP, or monoclonal anti-NP antibodies, precipitated NP proteins from acutely and persistently infected cells with equal efficiency. When the NP in acutely or persistently infected cells was labelled with either 14C- or 3H-amino acids and subjected to two-dimensional gel analysis, significant charge differences were observed between the virus proteins. When measles virus-infected cells were examined for virus protein synthesis at 40 degrees C, although no change was found in acutely infected cells, NP was not detected in the persistent infection.
对持续感染的人细胞培养物中细胞内麻疹病毒蛋白的合成进行了研究。通过一维和二维聚丙烯酰胺凝胶电泳对放射免疫沉淀后的病毒诱导蛋白进行了分析。与用于引发感染的病毒诱导的核蛋白（NP）相比，在持续感染细胞中合成的麻疹病毒诱导核蛋白与麻疹病毒抗体（人恢复期血清）的结合能力降低。相比之下，针对原始病毒NP制备的单特异性兔血清或单克隆抗NP抗体，能以相同效率从急性和持续感染细胞中沉淀出NP蛋白。当用14C或3H氨基酸标记急性或持续感染细胞中的NP并进行二维凝胶分析时，观察到病毒蛋白之间存在明显的电荷差异。当在40℃检测麻疹病毒感染细胞的病毒蛋白合成时，虽然在急性感染细胞中未发现变化，但在持续感染中未检测到NP。
