Moyer S A, Baker S C, Horikami S M
Department of Immunology and Medical Microbiology, University of Florida College of Medicine, Gainesville 32610.
J Gen Virol. 1990 Apr;71 ( Pt 4):775-83. doi: 10.1099/0022-1317-71-4-775.
We have developed a cell-free system derived from measles virus-infected cells that supported the transcription and replication of measles virus RNA in vitro. The data suggest that tubulin may be required for these reactions, since an anti-beta-tubulin monoclonal antibody inhibited viral RNA synthesis and the addition of purified tubulin stimulated measles virus RNA synthesis in vitro. Tubulin may be a subunit of the viral RNA polymerase, since two different anti-tubulin antibodies, one specific for the beta- and another specific for the alpha-subunit of tubulin, coimmunoprecipitated the measles virus L protein as well as tubulin from extracts of measles virus-infected cells. Other experiments further implicated actin in the budding process during virus maturation, as there appeared to be a specific association of actin in vitro only with nucleocapsids that have terminated RNA synthesis, which is presumably a prerequisite to budding.
我们开发了一种源自感染麻疹病毒细胞的无细胞系统，该系统在体外支持麻疹病毒RNA的转录和复制。数据表明，微管蛋白可能是这些反应所必需的，因为抗β-微管蛋白单克隆抗体抑制病毒RNA合成，而添加纯化的微管蛋白可在体外刺激麻疹病毒RNA合成。微管蛋白可能是病毒RNA聚合酶的一个亚基，因为两种不同的抗微管蛋白抗体，一种对微管蛋白的β亚基具有特异性，另一种对微管蛋白的α亚基具有特异性，可从感染麻疹病毒的细胞提取物中共免疫沉淀麻疹病毒L蛋白以及微管蛋白。其他实验进一步表明肌动蛋白在病毒成熟过程的出芽过程中起作用，因为在体外似乎只有肌动蛋白与已终止RNA合成的核衣壳有特异性关联，这大概是出芽的先决条件。
