ICAR - Krishi Vigyan Kendra, Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Kattupakkam, Chennai, Tamil Nadu, 603 203, India.
Crustacean Culture Division, ICAR - Central Institute of Brackishwater Aquaculture (CIBA), Chennai, Tamil Nadu, 600 028, India.
Curr Microbiol. 2023 Jul 18;80(9):290. doi: 10.1007/s00284-023-03372-3.
Grow-out and hatchery units of shrimps are being impacted by disease-causing bacterial pathogens and predominantly marine Vibrios. The use of chemicals for governing bacterial pathogens in the aquaculture practices is developing resistance to bacteria. Henceforth, the application of bio-therapeutic agents from marine resources for controlling pathogens is most vital to be considered. Molecular docking is computer-assisted drug design tool to detect and counteract for drug-receptor interaction for known target protein of diseases. Therefore, an effort was made with the extract of the marine micro alga Skeletonema costatum against hemolysin protein of pathogenic bacteria Vibrio harveyi. The extract of S. costatum was tested against growth and virulence produced by V. harveyi during larviculture of Penaeus monodon. The extract was analyzed for phyto-constituents through GC-MS and used them as ligand molecule in docking. S. costatum extract at 200 µg mL was found to decrease 35.20% of cumulative percentage mortality (CPM) in postlarvae of P. monodon against V. harveyi infections. The biomolecule Docasane, an alkane from the extract of S. costatum, exposed highest binding interaction than other compounds during docking analysis. The level of significance (P < 0.05) was found in CPM, growth, and virulence factors of V. harveyi studies. Thus, the present finding predicts that extract of S. costatum containing biomolecules can be recommended for use in the shrimp culture-based grow-out and hatchery units for eliminating bioluminescent V. harveyi.
虾类养殖和孵化单位受到致病细菌病原体和主要海洋弧菌的影响。在水产养殖实践中使用化学品来控制细菌病原体的做法正在导致细菌产生抗药性。因此，从海洋资源中应用生物治疗剂来控制病原体是至关重要的。分子对接是一种计算机辅助药物设计工具，用于检测和对抗已知疾病靶蛋白的药物-受体相互作用。因此，我们努力从海洋微藻 Skeletonema costatum 中提取物质来对抗致病性细菌 Vibrio harveyi 的溶血蛋白。在对斑节对虾幼体进行养殖期间，我们测试了 S. costatum 提取物对 V. harveyi 生长和产生的毒力的影响。通过 GC-MS 分析提取物中的植物成分，并将其用作对接中的配体分子。在斑节对虾幼体中，S. costatum 提取物在 200 µg mL 时被发现可将累积死亡率（CPM）降低 35.20%，从而抵抗 V. harveyi 的感染。在对接分析中，Docasane 是来自 S. costatum 提取物的烷烃，其表现出比其他化合物更高的结合相互作用。在 V. harveyi 的 CPM、生长和毒力因子研究中发现了显著水平（P < 0.05）。因此，目前的发现表明，含有生物分子的 S. costatum 提取物可推荐用于虾类养殖的养殖和孵化单位，以消除发光的 V. harveyi。
