School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo 255049, China.
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo 255049, China.
Food Chem. 2023 Dec 15;429:136857. doi: 10.1016/j.foodchem.2023.136857. Epub 2023 Jul 13.
At present, due to the coexistence of multiple pesticides in vegetables and the enhanced toxicity, a simultaneous detection method for multiple pesticides is urgently needed. In this work, two types of core-shell nanomaterials, Ag-Au core-shell nanoparticles (Ag@Au NPs) and CuO-Au core-shell nanoparticles (CuO@Au NPs), were synthesized and labeled with acetamiprid aptamer and malathion aptamer to prepare two novel electroactive signal probes, respectively. The two probes were hybridized on the surface of the electrode by the principle of base complementary pairing between the aptamers and the thiolated DNA oligonucleotide sequences, and a dual-signal electrochemical aptasensor for the simultaneous detection of acetamiprid and malathion was established by modified glassy carbon electrode (GCE). The limits of detection (LOD) were calculated to be 43.7 pg mL for acetamiprid and 63.4 pg mL for malathion. The aptasensor determined acetamiprid and malathion in spinach and rape with the recovery rates of 88.9%-112.5% and 98.0%-114.1%, respectively.
目前，由于蔬菜中多种农药并存且毒性增强，因此迫切需要一种同时检测多种农药的方法。在这项工作中，合成了两种核壳纳米材料，Ag-Au 核壳纳米粒子（Ag@Au NPs）和 CuO-Au 核壳纳米粒子（CuO@Au NPs），并分别用啶虫脒适体和马拉硫磷适体标记，制备了两种新型的电活性信号探针。这两种探针通过适体与巯基化 DNA 寡核苷酸序列之间的碱基互补配对原理在电极表面杂交，并通过修饰玻碳电极（GCE）建立了用于同时检测啶虫脒和马拉硫磷的双信号电化学适体传感器。计算出啶虫脒和马拉硫磷的检测限（LOD）分别为 43.7 pg mL 和 63.4 pg mL。该适体传感器用于测定菠菜和油菜中的啶虫脒和马拉硫磷，回收率分别为 88.9%-112.5%和 98.0%-114.1%。
