基于高多孔金的阻抗适体传感器用于水果和蔬菜中吡虫啉的灵敏检测。
Impedimetric aptasensor based on highly porous gold for sensitive detection of acetamiprid in fruits and vegetables.
机构信息
Institute of Agricultural Engineering, School of Agricultural Equipment and Engineering, Jiangsu University, Zhenjiang 212013, China.
Institute of Agricultural Engineering, School of Agricultural Equipment and Engineering, Jiangsu University, Zhenjiang 212013, China.
出版信息
Food Chem. 2020 Aug 30;322:126762. doi: 10.1016/j.foodchem.2020.126762. Epub 2020 Apr 6.
A novel electrochemical aptasensor modified with highly porous gold and aptamer was prepared for the determination of acetamiprid in fruits and vegetables. Highly porous gold was synthesized by electroreduction at -4 V in an electrolyte containing 2.5 mol/L NHCl and 10 mmol/L HAuCl. Acetamiprid-binding aptamer was immobilized on highly porous gold by self-assembly. Acetamiprid could be captured by aptamer on the sensing interface, resulting in an increment of electron transfer resistance. Thanks to the large specific surface area of highly porous gold and the high affinity of aptamer, the aptasensor exhibited a highly sensitive impedance response for acetamiprid. Under optimal condition, the aptasensor displayed a linear response for acetamiprid in the concentration range of 0.5-300 nmol/L, and the detection limit was 0.34 nmol/L. Furthermore, the aptasensor showed high selectivity, good reproducibility and stability. Finally, the aptasensor was applied for the determination of acetamiprid in fruits and vegetables with satisfactory results.
一种新型电化学适体传感器，通过在含有 2.5 mol/L NHCl 和 10 mmol/L HAuCl 的电解液中于-4 V 下进行电还原合成得到高度多孔金，并用其对金进行修饰，用于测定水果和蔬菜中的乙酰甲胺磷。通过自组装将与乙酰甲胺磷结合的适体固定在高度多孔金上。在传感界面上，乙酰甲胺磷可以被适体捕获，从而导致电子转移电阻增加。由于高度多孔金的大比表面积和适体的高亲和力，该适体传感器对乙酰甲胺磷表现出高灵敏度的阻抗响应。在最佳条件下，该适体传感器对 0.5-300 nmol/L 浓度范围内的乙酰甲胺磷呈现线性响应，检测限为 0.34 nmol/L。此外，该适体传感器表现出高选择性、良好的重现性和稳定性。最后，该适体传感器用于测定水果和蔬菜中的乙酰甲胺磷，结果令人满意。
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