Magee-Women's Research Institute, Department of Obstetrics, Gynecology & Reproductive Sciences, University of Pittsburgh, Pittsburgh, PA, USA.
Methods Mol Biol. 2023;2677:185-201. doi: 10.1007/978-1-0716-3259-8_11.
The mammalian reproductive cycle, including those of humans and mice, begins very early in development. In utero, the ovaries become populated with primordial germ cells (PGCs) that will generate the oogonia. First, these cells proliferate mitotically, and then they trigger the meiotic program and initiate meiotic prophase I. Since these processes happen during gestation, their study had been very limited and challenging. Recently, we reported that, in the naked mole-rat (Heterocephalus glaber) ovary, there is mitotic expansion of the PGCs, and the initiation of the meiotic program occurs postnatally. In this chapter, we present a comprehensive collection of protocols that permit the analysis of naked mole-rat germ cells, from PGCs to oocytes, in meiotic prophase I, using in vivo and in vitro approaches.
哺乳动物的生殖周期，包括人类和小鼠的生殖周期，早在发育早期就开始了。在子宫内，卵巢中充满了原始生殖细胞（PGCs），这些细胞将产生卵原细胞。首先，这些细胞通过有丝分裂增殖，然后它们触发减数分裂程序并开始减数分裂前期 I。由于这些过程发生在妊娠期，因此它们的研究非常有限且具有挑战性。最近，我们报道称，在裸鼹鼠（Heterocephalus glaber）的卵巢中，PGCs 有丝分裂扩张，减数分裂程序在出生后开始。在本章中，我们提供了一套全面的方案，允许使用体内和体外方法分析从 PGC 到减数分裂前期 I 的卵母细胞的裸鼹鼠生殖细胞。
