Bastide Grégoire M G B H, Remund Anna L, Oosthuizen Dina N, Derron Nina, Gerber Philipp A, Weber Ines C
Particle Technology Laboratory, Department of Mechanical and Process Engineering, ETH Zurich CH-8092 Zurich Switzerland
Department of Endocrinology, Diabetology, and Clinical Nutrition, University Hospital Zurich (USZ) and University of Zurich (UZH) CH-8091 Zurich Switzerland.
Sens Diagn. 2023 Jun 27;2(4):918-928. doi: 10.1039/d3sd00079f. eCollection 2023 Jul 17.
Non-invasive breath analysis with mobile health devices bears tremendous potential to guide therapeutic treatment and personalize lifestyle changes. Of particular interest is the breath volatile acetone, a biomarker for fat burning, that could help in understanding and treating metabolic diseases. Here, we report a hand-held (6 × 10 × 19.5 cm), light-weight (490 g), and simple device for rapid acetone detection in breath. It comprises a tailor-made end-tidal breath sampling unit, connected to a sensor and a pump for on-demand breath sampling, all operated using a Raspberry Pi microcontroller connected with a HDMI touchscreen. Accurate acetone detection is enabled by introducing a catalytic filter and a separation column, which remove and separate undesired interferents from acetone upstream of the sensor. This way, acetone is detected selectively even in complex gas mixtures containing highly concentrated interferents. This device accurately tracks breath acetone concentrations in the exhaled breath of five volunteers during a ketogenic diet, being as high as 26.3 ppm. Most importantly, it can differentiate small acetone changes during a baseline visit as well as before and after an exercise stimulus, being as low as 0.5 ppm. It is stable for at least four months (122 days), and features excellent bias and precision of 0.03 and 0.6 ppm at concentrations below 5 ppm, as validated by proton-transfer-reaction time-of-flight mass spectrometry (PTR-ToF-MS). Hence, this detector is highly promising for simple-in-use, non-invasive, and routine monitoring of acetone to guide therapeutic treatment and track lifestyle changes.
利用移动健康设备进行非侵入式呼吸分析在指导治疗和个性化生活方式改变方面具有巨大潜力。特别值得关注的是呼吸挥发性丙酮，它是脂肪燃烧的生物标志物，有助于理解和治疗代谢疾病。在此，我们报告一种手持式（6×10×19.5厘米）、轻便（490克）且简单的设备，用于快速检测呼吸中的丙酮。它包括一个定制的潮气末呼吸采样单元，连接到一个传感器和一个按需呼吸采样泵，所有这些都通过与HDMI触摸屏相连的树莓派微控制器进行操作。通过引入催化过滤器和分离柱实现了丙酮的准确检测，它们在传感器上游去除并分离丙酮中不需要的干扰物。这样，即使在含有高浓度干扰物的复杂气体混合物中也能选择性地检测到丙酮。该设备准确跟踪了五名志愿者在生酮饮食期间呼出气体中的呼吸丙酮浓度，高达26.3 ppm。最重要的是，它能够区分基线访视期间以及运动刺激前后小至0.5 ppm的丙酮变化。它至少稳定四个月（122天），并且经质子转移反应飞行时间质谱（PTR-ToF-MS）验证，在浓度低于5 ppm时具有0.03和0.6 ppm的出色偏差和精度。因此，这种检测器在用于简单、非侵入性且常规监测丙酮以指导治疗和跟踪生活方式改变方面极具前景。
