Tian Rui, Li Huixin, Ren Songjie, Li Shukui, Fang Run, Liu Yang
Gynecology Department, Shanghai Mengchao Cancer Hospital, Shanghai 201800, China.
Urinary Surgery, Renhe Hospital, Baoshan District, Shanghai 200431, China.
Open Med (Wars). 2023 Jul 14;18(1):20230709. doi: 10.1515/med-2023-0709. eCollection 2023.
Circular RNA (circRNA) THBS1 has been shown to exist as an oncogene in non-small-cell lung cancer, but its role in cervical cancer is still unclear. Our experiment aimed to uncover the functions and specific mechanism of circRNA THBS1 in cervical cancer cells. Levels of circRNA THBS1 and miR-543 in cervical cancer tissues and cell lines were assessed by RT-qPCR. starBase and dual luciferase reporter gene assay were applied for investigating the correlation between miR-543 and circRNA THBS1/HMGB2. Cell proliferation and apoptosis were evaluated by MTT and flow cytometry, respectively. Furthermore, the levels of HMGB2, E-cadherin, and N-cadherin in HeLa cells were determined by RT-qPCR and western blot analysis. Our data revealed that circRNA THBS1 was significantly upregulated and miR-543 was low expressed in cervical cancer tissues and cell lines. circRNA THBS1 interacted with miR-543 and negatively regulated miR-543 expression in HeLa cells. Silencing of circRNA THBS1 remarkably suppressed HeLa cells' viability, accelerated cells' apoptosis, and inhibited the EMT of HeLa cells, while these changes were reversed by miR-543 inhibitor. Moreover, miR-543 affected HeLa cells by targeting HMGB2. In conclusion, circRNA THBS1 silencing inhibited the malignant biological behaviors of cervical cancer cells via the regulation of miR-543/HMGB2 axis.
环状RNA（circRNA）THBS1已被证明在非小细胞肺癌中作为一种癌基因存在，但其在宫颈癌中的作用仍不清楚。我们的实验旨在揭示circRNA THBS1在宫颈癌细胞中的功能和具体机制。通过RT-qPCR评估宫颈癌组织和细胞系中circRNA THBS1和miR-543的水平。应用starBase和双荧光素酶报告基因检测来研究miR-543与circRNA THBS1/HMGB2之间的相关性。分别通过MTT和流式细胞术评估细胞增殖和凋亡。此外，通过RT-qPCR和蛋白质免疫印迹分析测定HeLa细胞中HMGB2、E-钙黏蛋白和N-钙黏蛋白的水平。我们的数据显示，circRNA THBS1在宫颈癌组织和细胞系中显著上调，而miR-543低表达。circRNA THBS1与miR-543相互作用并负向调节HeLa细胞中miR-543的表达。沉默circRNA THBS1显著抑制HeLa细胞的活力，加速细胞凋亡，并抑制HeLa细胞的上皮-间质转化，而这些变化被miR-543抑制剂逆转。此外，miR-543通过靶向HMGB2影响HeLa细胞。总之，circRNA THBS1沉默通过调节miR-543/HMGB2轴抑制宫颈癌细胞的恶性生物学行为。
