一氧化氮合酶 2 基因变异 rs2297514 与汉族人群四肢创伤后骨髓炎易感性降低相关。
Nitric oxide synthase 2 genetic variation rs2297514 associates with a decreased susceptibility to extremity post-traumatic osteomyelitis in a Chinese Han population.
机构信息
Division of Orthopaedics and Traumatology, Department of Orthopaedics, Nanfang Hospital, Southern Medical University, Guangzhou, China.
Guangdong Provincial Key Laboratory of Bone and Cartilage Regenerative Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China.
出版信息
Front Cell Infect Microbiol. 2023 Jul 3;13:1177830. doi: 10.3389/fcimb.2023.1177830. eCollection 2023.
BACKGROUND
Previous studies have indicated that nitric oxide synthase 2 () genetic variations are involved in delayed fracture healing and fracture non-union. Whether these genetic variants associate with the development of osteomyelitis (OM) remains unclear. Here, we analyzed the potential relationships between genetic variations and the risk of developing post-traumatic OM (PTOM) in a Chinese Han population.
METHODS
Altogether 704 participants, including 336 PTOM patients and 368 healthy controls, were genotyped of rs2297514 and rs2248814 of the gene using the SNaPshot genotyping method.
RESULTS
Outcomes showed that the frequency of allele C of rs2297514 in the patient group was significantly lower than that in the control group (48.7% . 54.5%, = 0.029, OR = 0.792, 95% CI 0.642 - 0.976). In addition, significant associations were found between rs2297514 and susceptibility to PTOM by the recessive model ( = 0.007, OR = 0.633, 95% CI 0.453 - 0.884), and the homozygous model ( = 0.039, OR = 0.648, 95% CI 0.429 - 0.979). Moreover, patients with the CC genotype of rs2297514 had lower inflammatory biomarkers levels than the TT genotype, especially for the C-reactive protein (CRP) level (median: 4.1 mg/L . 8.9 mg/L, = 0.027). However, no significant relationship was noted between rs2248814 and the risk of developing PTOM.
CONCLUSION
In this Chinese cohort, rs2297514 is correlated with a decreased risk of PTOM development, with genotype CC as a protective factor.
背景
先前的研究表明，一氧化氮合酶 2 () 基因变异与延迟骨折愈合和骨折不愈合有关。这些遗传变异是否与骨髓炎 (OM) 的发生有关尚不清楚。在这里，我们分析了中国汉族人群中基因变异与创伤后 OM (PTOM) 发展风险之间的潜在关系。
方法
共纳入 704 名参与者，包括 336 例 PTOM 患者和 368 例健康对照者，采用 SNaPshot 基因分型法对基因 rs2297514 和 rs2248814 进行基因分型。
结果
结果显示，患者组 rs2297514 等位基因 C 的频率明显低于对照组（48.7%. 54.5%，= 0.029，OR = 0.792，95%CI 0.642 - 0.976）。此外，rs2297514 与 PTOM 的易感性呈隐性模型相关（= 0.007，OR = 0.633，95%CI 0.453 - 0.884），以及纯合模型（= 0.039，OR = 0.648，95%CI 0.429 - 0.979）。此外，rs2297514 中 CC 基因型的患者的炎症生物标志物水平低于 TT 基因型，尤其是 C 反应蛋白（CRP）水平（中位数：4.1 mg/L. 8.9 mg/L，= 0.027）。然而，rs2248814 与 PTOM 的发病风险无显著相关性。
结论
在这个中国队列中，rs2297514 与 PTOM 发病风险降低相关，CC 基因型为保护因素。
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