Graduate Program in Dentistry, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
Braz Dent J. 2023 May-Jun;34(3):42-49. doi: 10.1590/0103-6440202305244.
The present study aimed to evaluate bacterial viability after the use of different disinfection protocols in root canals infected with a multispecies biofilm (MB) formed in situ. Palatal roots with a single canal were obtained from extracted maxillary molars and sterilized before being inserted into the mouth. The roots were contaminated with a MB in an intraoral appliance worn by ten volunteers. All volunteers wore six roots simultaneously in two intraoral devices for 21 days. One root from each volunteer was assigned to each group (n=10): PUI - passive ultrasonic irrigation; EC - Easy Clean; XPF - XP-endo Finisher; aPDT - antimicrobial photodynamic therapy; CI - conventional irrigation; and NC - negative control. The samples were evaluated under confocal laser scanning microscopy. The percentage of viable cells (VC) was calculated over the total percentage of MB biovolume. Data were statistically analyzed (α=5%). The cell viability in the entire root canal or for each third was compared between groups (Kruskal-Wallis test, Dunn post-hoc test) and for the same group (Friedman test, Dunn post-hoc test). Disinfection protocols were not significantly different from each other (P>.05). Samples in EC, PUI, and aPDT had lower cell viability than in NC (P<.05). In the coronal third of samples in the EC, XPF, PUI and aPDT, the percentage of VC biovolume was lower than in the NC (P<.05). The percentage of VC in EC samples was lower in the coronal and middle thirds than in the apical third (P<.05). EC, PUI and aPDT had significant effects on cell viability in intraradicular multispecies biofilm formed in situ when compared with untreated samples.
本研究旨在评估使用不同消毒方案后，感染多物种生物膜（MB）的根管内细菌活力。腭根具有一个单一的通道，从提取的上颌磨牙获得，并在插入口腔之前进行消毒。根通过十位志愿者佩戴的口腔内设备中的 MB 污染。所有志愿者同时在两个口腔内设备中佩戴六个根 21 天。每位志愿者的一根根被分配到每组（n=10）：PUI - 被动超声冲洗；EC - 轻松清洁；XPF - XP-endo 终结者；aPDT - 抗菌光动力疗法；CI - 常规冲洗；和 NC - 阴性对照。使用共聚焦激光扫描显微镜评估样品。活细胞（VC）的百分比根据 MB 生物量的总百分比计算。数据进行了统计学分析（α=5%）。通过 Kruskal-Wallis 检验和 Dunn 事后检验比较各组之间（Kruskal-Wallis 检验，Dunn 事后检验）和同一组之间（Friedman 检验，Dunn 事后检验）整个根管或每三分之一的细胞活力。消毒方案彼此之间没有显着差异（P>.05）。与 NC 相比，EC、PUI 和 aPDT 中的样本具有更低的细胞活力（P<.05）。在 EC、XPF、PUI 和 aPDT 样本的冠部三分之一中，VC 生物量的百分比低于 NC（P<.05）。与根尖部相比，EC 样本的 VC 百分比在冠部和中部较低（P<.05）。与未处理的样本相比，EC、PUI 和 aPDT 对原位形成的根管内多物种生物膜的细胞活力有显着影响。
