Department of Basic and Oral Biology, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
Braz Dent J. 2023 May-Jun;34(3):50-56. doi: 10.1590/0103-6440202304980.
Osteoporosis can affect a significant part of the population and fractures are the most common complications associated with this disease, leading to high public health costs. Thus, the prevention of fractures is relevant to individuals with signs and symptoms as well as to the health system. Postmenopausal osteoporosis has been associated with oxidative stress, emphasizing the importance of an efficient defense system to maintain bone health. Lycopene is a carotenoid with antioxidant properties that may stimulate osteoblastogenesis and inhibit osteoclastogenesis. The purpose of this investigation was to analyze the influence of lycopene in the bone neoformation of calvaria defects in ovariectomized rats utilizing the concentration of 45 mg/kg. Wistar Hannover female rats were divided into ovariectomized and sham groups. The ovariectomized animals received 45 mg/kg lycopene (OvxL) or water (Ovx) by daily gavage the day after ovariectomy/sham surgery for 16 weeks. Twelve weeks after ovariectomy, there were performed 5-mm calvaria defects followed by euthanasia after 4 weeks. Samples of bone tissue were collected to perform morphological and morphometrical analysis of the neoformed bone area, and percentage with Software Image J. Morphological evaluation showed mature bone with more osteocytes in the group OVxL when compared to the other groups. The morphometrical analysis demonstrated a significant increase of bone neoformation in the group OvxL (p<0.05). The data obtained suggest that lycopene benefits bone repair in the absence of estrogenic hormones.
骨质疏松症可影响相当一部分人群，骨折是该病最常见的并发症，导致高额公共卫生费用。因此，预防骨折不仅与有相关症状和体征的个体有关，也与卫生系统有关。绝经后骨质疏松症与氧化应激有关，这强调了维持骨骼健康的有效防御系统的重要性。番茄红素是一种具有抗氧化特性的类胡萝卜素，可能刺激成骨细胞生成并抑制破骨细胞生成。本研究旨在分析番茄红素对去卵巢大鼠颅骨缺损新骨形成的影响，使用浓度为 45mg/kg。Wistar Hannover 雌性大鼠分为去卵巢组和假手术组。去卵巢动物在去卵巢/假手术术后第 1 天每天通过灌胃接受 45mg/kg 番茄红素（OvxL）或水（Ovx），持续 16 周。去卵巢后 12 周，进行 5mm 颅骨缺损，4 周后安乐死。收集骨组织样本，使用 Image J 软件进行新形成骨面积和百分比的形态学和形态计量学分析。形态学评估显示，与其他组相比，Ovxl 组的成熟骨中具有更多的骨细胞。形态计量学分析表明 Ovxl 组的新骨形成显著增加（p<0.05）。研究结果表明，在缺乏雌激素的情况下，番茄红素有益于骨骼修复。
